Our StemTAG™ 96-Well Stem Cell Colony Formation Assay provides a high-throughput method to quantify ES cells in just 7-10 days, and no manual cell counting is required. Once colonies are formed, they may be analyzed in three different ways:

1. Lyse cells, then quantify in a fluorescence plate reader using dye included in the kit.

2. Lyse cells, then quantify alkaline phosphatase activity using reagents provided.

3. Recover colonies from matrix for further culture or analysis.

Figure 1: Assay Principle. Cells may be either lysed and quantified or recovered for further downstream analysis.

Figure 2: Anchorage-Independent Growth of Mouse ES-D3 Cells. Alkaline phosphatase staining.