The AAV Helper Free System for produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1).
Figure 1: Production and Transduction of AAV2-GFP. AAV2-GFP was produced by cotransfection of 293AAV cells (Catalog #AAV-100) with pAAV-GFP, pAAV-RC2, and pHelper plasmids. Upon harvesting viral supernatant, 293AD cells (Catalog #AD-100) were infected with AAV2-GFP for 48 hours.
Figure 2: Production and Transduction of AAV2-GFP. Top Left: 293AAV cells prior to transfection (10X). Top Right: 293AAV cells 48 hours after transfection (10X). Bottom Left: GFP expression in 293AAV cells 48 hours after transfection (10X). Bottom Right: GFP expression in 293AD cells 48 hours after infection (20X).
Figure 3: Generation of AAV-DJ through Capsid DNA Family Shuffling.