AAV Helper Free Expression SystemsAAV Expression and Packaging
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- pHelper plasmid contains required E2A, E4, and VA RNA adenoviral genes; eliminates the need for a helper adenovirus
- Complete Expression Systems contain packaging plasmids, expression vector and GFP control vector
- AAV-DJ system provides a hybrid capsid created from 8 different AAV serotypes to provide substantially higher infectivity rates across a broad range of tissues
- AAV-DJ/8 is a mutant of AAV-DJ designed to provide increased uptake in brain tissue, closely mimicking AAV-8 and AAV-9
NOTE: AAV-DJ and AAV-DJ/8 Helper Free Systems are available for sale to academic, government and non-profit research laboratories. All other purchasers require a commercial license for all fields including research use. Please contact our Business Development department for license information.
The AAV Helper Free System for produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1).
AAV Helper Free Expression Systems are available for native serotypes 1 through 6, as well as the novel AAV-DJ and AAV-DJ/8. The AAV-DJ system provides a hybrid capsid created by DNA shuffling technology combining 8 different native serotypes: AAV-2, AAV-4, AAV-5, AAV-8, AAV-9, avian AAV, bovine AAV, and caprine AAV. The result is a highly infectious vector that can transduce a wide variety of cells and tissues at significantly higher rates than AAV-21.
AAV-DJ/8 was created by making a point mutation in the heparin binding domain of AAV-DJ. Recombinant AAV produced from the AAV-DJ/8 system closely resembles AAV-8 and AAV-9 in its ability to infect heart and brain tissues.
1. Grimm, D. et al. (2008). J. Virol. 82: 5887-5911.
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Figure 1: Production and Transduction of AAV2-GFP. AAV2-GFP was produced by cotransfection of 293AAV cells (Catalog #AAV-100) with pAAV-GFP, pAAV-RC2, and pHelper plasmids. Upon harvesting viral supernatant, 293AD cells (Catalog #AD-100) were infected with AAV2-GFP for 48 hours.
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Figure 2: Production and Transduction of AAV2-GFP. Top Left: 293AAV cells prior to transfection (10X). Top Right: 293AAV cells 48 hours after transfection (10X). Bottom Left: GFP expression in 293AAV cells 48 hours after transfection (10X). Bottom Right: GFP expression in 293AD cells 48 hours after infection (20X).
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Figure 3: Generation of AAV-DJ through Capsid DNA Family Shuffling.
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