AAV Quantitation / Titer

Adeno-Associated Virus (AAV) Expression

Ultra-fast procedure (<2 hours)
Measures the viral nucleic acid content of unpurified AAV-2 or AAV-DJ supernatant or purified AAV-1, -2 or -5
Limit of detection: 10^9 GC/mL from unpurified AAV supernatant or 5 x 10^10 GC/mL for purified AAV

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Product Details
Manuals/MSDS

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Product Name	Catalog Number	Size	Detection	Qty.	Price
QuickTiter™ AAV Quantitation Kit	VPK-145	20 assays	Fluorometric		Select Country

Overview

Traditionally, AAV particles have been quantified by DNA dot blot or similar procedures. These methods can be time-consuming and suffer from a high degree of inter-assay variability.

Our QuickTiter™ AAV Quantitation Kit uses a proprietary technology to quantify the viral nucleic acid content of AAV preps. The kit can be used with unpurified supernatant of AAV-2 or AAV-DJ, or with purified AAV-1, AAV-2 or AAV-5.

Figures

Figure 1: Assay Principle for the QuickTiter™ AAV Quantitation Kit.

Figure 1

Figure 2: Standard Curve Generated with the QuickTiter™ AAV Quantitation Kit.The QuickTiter™ AAV-2 DNA Standard was diluted as described in the Assay Protocol. Fluorescence measurement was performed on a SpectraMax Gemini XS Fluorometer (Molecular Devices) with a 485/538 nm filter set and 530 nm cutoff.

Figure 2

Product Name	Catalog Number	Manual / Data Sheet	MSDS
QuickTiter™ AAV Quantitation Kit	VPK-145	Download	Download

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