Cell Biolabs’Aflatoxin Competitive ELISA Kit provides a convenient method for the detection of total AflatoxinB1 and AflatoxinB2 adducts in protein samples. First, an AFB1 conjugate is coated on an ELISA plate. The unknown AFB1/2 samples or AFB1-BSA standards are then added to the AFB1 conjugate preabsorbed ELISA plate. After a brief incubation, an anti-AFB1/2 monoclonal antibody is added, followed by an HRP conjugated secondary antibody. The total content of AFB1 plus AFB2 in unknown samples is determined by comparison with a predetermined AFB1-BSA standard curve.