Alkaline Phosphatase (AP) is a widely used marker for both mouse and human embryonic stem cells (ES) and embryonic germ cells (EG). Our StemTAG™ Alkaline Phosphatase kits provide an efficient system for monitoring cell differentiation or undifferentiation using the AP marker.
The StemTAG™ Alkaline Phosphatase Activity Assay Combo Kits provide reagents for monitoring alkaline phosphatase activity via immunocytochemistry staining as well as in a 96-well plate with either colorimetric or fluorescence detection.
Figure 1: Staining of Undifferentiated and Differentiated ES cells using the StemTAG™ Alkaline Phosphatase Staining Kit. Murine embryonic stem cells (ES-D3) were maintained in an undifferentiated state on gelatin-coated dishes in the presence of LIF. To induce differentiation, LIF was withdrawn over a period of several days; various differentiation events were observed (cells became flattened and enlarged with reduced proliferation). At the end of day 5, cells were stained according to the StemTAG™ Alkaline Phosphatase Staining Kit assay protocol.