The CytoSelect™ 8-Channel Endothelial Biochip is a self-contained unit containing 8 channels with an inlet/outlet port at both ends of each channel. The channels of the 8-Channel Endothelial Biochip are first coated with one or more ligands, e.g. adhesion molecules such as collagen, fibrinogen, by dispensing the ligand into one of the ports. After an incubation period, endothelial cells such as HUVEC are added. Then the cells to be assayed are added using a microfluidic syringe pump at a specified flow rate that mimics in vivo shear stresses. Cell adhesion may be visualized by brightfield or phase contrast microscopy.
