The Global DNA Methylation ELISA Kit is a competitive ELISA for the quantitative measurement of 5MedCyd. The unknown 5MedCyd samples or 5MedCyd standards are first added to a 5MedCyd DNA conjugate coated EIA plate. After a brief incubation, an anti-5MedCyd monoclonal antibody is added, followed by an HRP conjugated secondary antibody. The 5MedCyd content in unknown samples is determined by comparison with a predetermined 5MedCyd standard curve.
Our Global DNA Hydroxymethylation ELISA Kit (5-Hydroxymethylcytosine Quantitation) is an enzyme immunoassay developed for rapid detection and quantitation of 5-hmC in any DNA samples. The quantity of 5-hmC in unknown sample is determined by comparing its absorbance with that of a known hydroxymethylated DNA standard curve.
