GST Inclusion Body Refolding | Cell Biolabs, Inc.

GST Inclusion Body Refolding

Cell Signaling and Protein Biology

Quickly solubilize and renature GST inclusion bodies
No lengthy dialysis or dilution steps
No pH variation or redox pair involved

Ordering
Product Details
Documents
Citations

Please select your country:

Product Name	Catalog Number	Size	Detection	Qty.	Price
Rapid GST Inclusion Body Solubilization and Renaturation Kit	AKR-110	1 kit	N/A		Select Country

Overview

Recombinant proteins expressed in bacteria often form inclusion bodies, especially when they are expressed at high levels. The Rapid GST Inclusion Body Solubilization and Renaturation Kit is designed to retrieve expressed GST fusion proteins in soluble form after lysis and extraction procedures. Each kit contains sufficient reagents to solubilize and renature up to 5-10 liters of bacterial culture.

Figures

Figure 1: GST Inclusion Body Solubilization and Renaturation Flow Chart

Figure 1

Figure 2: Solubilization and Renaturation of GST-RTK fusion protein. GST-RTK expression was induced with 1 mM IPTG at 37ºC for 4 hours. The cell pellet was lysed, and inclusion bodies were solubilized and renatured under different amounts of detergent solubilization solution according to the assay protocol. Lane 1: MW Standard; Lane 2: Whole E. coli lysate; Lanes 3, 7, 11: No detergent; Lanes 4, 8, 12: 32-fold dilution; Lanes 5, 9, 13: 8-fold dilution; Lanes 6, 10, 14: 2-fold dilution.

Figure 2

Product Name	Catalog Number	Manual / Data Sheet	MSDS
Rapid GST Inclusion Body Solubilization and Renaturation Kit	AKR-110	Download	Download

	Cdc42 Activation Assay PAK1
	Raf-1 RBD Agarose Beads Pull down only the active form of Ras prot...
	RhoA/Rac1/Cdc42 Activation Assay Combo Kit Contains reagents for 10 assays each of RhoA