iPS Cell Reprogramming

iPS Cell Reprogramming

Recent studies have demonstrated the ability to reprogram adult fibroblasts into pluripotent stem cells by introducing key factors using viral vector technologies. Such induced pluripotent stem cells (iPS cells) hold much promise for the advancement of stem cell research.

The breakthrough research was performed in the laboratory of Nobel-prize winner Dr. Shinya Yamanaka* at Kyoto University using retrovirus to introduce the key stem cell factors. Later studies used an alternate set of factors. We offer the same retroviral packaging cell lines and stem cell factors used in these pioneering studies.

A more recent advance has been the development of polycistronic iPS plasmids, a single vector which contains a fusion of 4 genes: Klf4, Oct3/4, Sox2 and c-Myc. This plasmid allows iPS cells to be generated with just a single viral vector rather than several individual viruses each containing one gene.

*Yamanaka, et. al (2006). Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 126(4):663-676.

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