Lentiviral expression has many advantages over other viruses, including the ability to infect both proliferating and non-proliferating cells. The efficiency of lentivirus infection of target cells, however, tends to be lower. Additives such as Polybrene® can increase transduction efficiencies, but even then only a small fraction of lentiviral vectors can trasduce many target cell lines.
Our ViraDuctin™Lentivirus Transduction Kit provides superior lentiviral transduction efficiencies in a variety of cell lines, even when compared to transductions in the presence of Polybrene®. This system is ideal for many primary cells as well as immobilized cells.
*Note: The number of transductions per kit is based on use of a 24-well plate. The kit may also be used with 96-well, 12-well or 6-well plates, as well as 60 mm or 100 mm dishes. Please see product manual for more details.
Figure 1: Transduction of 293AD and HT-1080 Cells. 293AD cells (#AD-100) and HT-1080 cells were each seeded at 50,000 cells/well in a 24-well plate overnight. Cells were infected with GFP lentivirus for 48 hours in the presence of no additive (left), Polybrene® (middle), or the ViraDuctin™ Lentivirus Transduction Kit (right).
Figure 2: Transduction Efficiencies in Various Cell Lines. NIH3T3 cells, HeLa cells, our own 293AD cells (#AD-100), and HT-1080 cells were each seeded at 50,000 cells/well in a 24-well plate overnight. Cells were infected with GFP lentivirus for 48 hours in the presence of Polybrene® or the ViraDuctin™ Lentivirus Transduction Kit. For each cell line, fluorescence levels using the ViraDuctin™ Lentivirus Kit are depicted relative to a normalized fluorescence level of 100 for transductions with Polybrene®.