Lipid Quantification Kit (unsaturated fatty acids)

Lipid Quantification Kit (unsaturated fatty acids)
  • Quantifies total unsaturated fatty acids from plasma, serum, or cultured cells
  • Sulfo-phospho-vanillin system provides quantitation in a 96-well plate
Email To BuyerPrint this PageCopy Link
Ordering

Please contact your distributor for pricing.

Lipid Quantification Kit (Colorimetric)
Catalog Number
STA-613
Size
100 assays
Detection
Colorimetric
Manual/Data Sheet Download
SDS Download
Price
$480.00
Product Details

Traditional lipid quantitation methods require expensive HPLC or light scattering detection equipment. Our Lipid Quantitation Kit provides a simple method for quantitation of lipids in a standard 96-well microplate reader. The kit uses a sulfo-phospho-vanillin system in which samples are acidified and heated to solubilize and prime them for quantitation. In an acidic environment, vanillin reagent reacts with the lipids to form a colored product that is easily detected by the plate reader at 540 nm.

Recent Product Citations
  1. Gao, H. et al. (2023). Outer membrane vesicles from a mosquito commensal mediate targeted killing of Plasmodium parasites via the phosphatidylcholine scavenging pathway. Nat Commun. 14(1):5157. doi: 10.1038/s41467-023-40887-6.
  2. Soetikno V. et al. (2023). Alpha-Mangosteen lessens high-fat/high-glucose diet and low-dose streptozotocin induced-hepatic manifestations in the insulin resistance rat model. Pharm Biol. 61(1):241-248. doi: 10.1080/13880209.2023.2166086.
  3. Barlin, M. et al. (2022). Proteins in Tumor-Derived Plasma Extracellular Vesicles Indicate Tumor Origin. Mol Cell Proteomics. 22(1):100476. doi: 10.1016/j.mcpro.2022.100476.
  4. Mansouri, S. et al. (2022). MPYS Modulates Fatty Acid Metabolism and Immune Tolerance at Homeostasis Independent of Type I IFNs. J Immunol. doi: 10.4049/jimmunol.2200158.
  5. Mohamed, M.F. et al. (2022). Presence of CrkI-containing microvesicles in squamous cell carcinomas could have ramifications on tumor biology and cancer therapeutics. Sci Rep. 12(1):4803. doi: 10.1038/s41598-022-08905-7.
  6. Barrett, E. et al. (2021). Uteroplacental Insufficiency with Hypoxia Upregulates Placental PPARγ-KMT5A Axis in the Rat. Reprod Sci. doi: 10.1007/s43032-020-00434-w.
  7. Kumar, S. et al. (2021). Cocaine-Specific Effects on Exosome Biogenesis in Microglial Cells. Neurochem Res. doi: 10.1007/s11064-021-03231-2.
  8. Pishavar, E. et al. (2020). Comparison Study of Stem Cell-Derived Extracellular Vesicles for Enhanced Osteogenic Differentiation. Tissue Eng Part A. doi: 10.1089/ten.TEA.2020.0194.
  9. Kawabe, Y. et al. (2020). (Bombyx Insulin-Like Peptide) Increases the Respiration Rate Through Facilitation of Carbohydrate Catabolism in Bombyx mori. Front Endocrinol (Lausanne). 10:150. doi: 10.3389/fendo.2019.00150.
  10. Inagaki, T. et al. (2020). Gut microbial pulse provides nutrition for parental provisioning in incipient termite colonies. Behav Ecol Sociobiol. 74(6). doi: 10.1007/s00265-020-02843-y.
  11. Zhu, Q. et al. (2020). Bupivacaine inhibits angiogenesis through oxidative stress-dependent inhibition of Akt/mTOR and activation of AMPK. Fundam Clin Pharmacol. doi: 10.1111/fcp.12554.
  12. Nair, P. et al. (2020). Metabolic responses to long-term food deprivation in subterranean and surface amphipods. Subterr Biol. 33:1–15. doi: 10.3897/subtbiol.33.48483.
  13. Langridge, T.D. et al. (2019). Toward understanding polymer micelle stability: Density ultracentrifugation offers insight into polymer micelle stability in human fluids. J Control Release. 319:157-167. doi: 10.1016/j.jconrel.2019.12.038.
  14. Ahmadpour, A. et al. (2019). Study of the Circulatory Energy Balance Indicators and Hepatic Fat Content in Dromedary Camel during Late Pregnancy and Early Lactation. Small Ruminant Research. doi: 10.1016/j.smallrumres.2019.08.018.
  15. Huang, Z. et al. (2019). ALOX12 inhibition sensitizes breast cancer to chemotherapy via AMPK activation and inhibition of lipid synthesis. Biochem Biophys Res Commun. pii: S0006-291X(19)30743-0. doi: 10.1016/j.bbrc.2019.04.101.
  16. Fanning, S. et al. (2019). Lipidomic Analysis of α-Synuclein Neurotoxicity Identifies Stearoyl CoA Desaturase as a Target for Parkinson Treatment. Mol Cell. 73(5):1001-1014.e8. doi: 10.1016/j.molcel.2018.11.028.
  17. Stranska, R. et al. (2018). Comparison of membrane affinity-based method with size-exclusion chromatography for isolation of exosome-like vesicles from human plasma. J Transl Med. 16(1):1. doi: 10.1186/s12967-017-1374-6.
  18. Yang, X. et al. (2018). Inhibiting 6-phosphogluconate dehydrogenase selectively targets breast cancer through AMPK activation. Clin Transl Oncol. 20(9):1145-1152. doi: 10.1007/s12094-018-1833-4.
  19. Tosic, M. et al. (2018). Lsd1 regulates skeletal muscle regeneration and directs the fate of satellite cells. Nat Commun. 9(1):366. doi: 10.1038/s41467-017-02740-5.
  20. Gupta, K.H. et al. (2017). Apoptosis and compensatory proliferation signaling are coupled by CrkI-containing microvesicles. Dev. Cell. 41(6):674-684.
  21. Tyszka-Czochara, M. et al. (2017). Caffeic Acid Expands Anti-Tumor Effect of Metformin in Human Metastatic Cervical Carcinoma HTB-34 Cells: Implications of AMPK Activation and Impairment of Fatty Acids De Novo Biosynthesis. Int J Mol Sci. doi: 10.3390/ijms.
  22. Tasaki, E. et al (2017). An Efficient Antioxidant System in a Long-Lived Termite Queen. PLoS One. 12(1):e0167412. doi: 10.1371/journal.pone.0167412.
  23. Duteil, D. et al. (2016). Lsd1 ablation triggers metabolic reprogramming of brown adipose tissue. Cell Reports 17:1008-1021.