MDA, or malondialdehyde, is a widely accepted marker for oxidative stress. Our OxiSelect™MDAImmunoblot Kit provides a specific method for detection of this lipid peroxidation by-product.
Important Note: MDA adducts are not stable long term. For best results test all samples immediately upon collection, or freeze them at -80ºC for up to one month. MDA may be degraded in samples that have been frozen for longer periods; in such cases more reliable results may be obtained from more stable markers of oxidative stress such as protein carbonyl, 8-OHdG or 4-HNE.
Figure 1: Immunoblotting with the OxiSelect™ MDA Immunoblot Kit. MDA-BSA Immunoblot Control was electroblotted onto a nitrocellulose membrane. MDA was detected using an anti-MDA antibody as described in the assay protocol.