Advanced glycation end products (AGE) are formed during the Maillard reaction where reducing carbohydrates react with lysine side chains and N-terminal amino groups of various macromolecules, particularly proteins (Figure 1). The advanced glycation end products can adversely affect the fuction of these macromolecules. One of the most prevalent AGE products, N-epsilon-(Carboxymethyl) Lysine, has been implicated in oxidative stress and vascular damage.
The OxiSelect™Methylglyoxal (MG) ELISA Kit detects and quantifies MG protein adducts. MG has been found to be involved in various pathological processes, for example, MG derivatives are found elevated in diabetes.
Figure 2: MG level in human serum and plasma samples. The protein concentrations of normal human serum and plasma were first determined by Pierce BCA Protein Assay and subsequently diluted to 10 μg/mL with 1X PBS (Undiluted Sample) or further diluted with Reduced BSA. Samples were then coated onto a 96-well Protein Binding Plate. The MG levels were determined as described in the Assay Protocol. Note: Undiluted plasma and serum samples result in saturation absorbance.