The AAV Helper Free System was developed to make production of AAV safer and more convenient. It absolutely eliminates the need for adenovirus transduction! Instead the adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) and in the 293 packaging cells (E1). The overall system includes 2 packaging vectors (pAAV-RC2 and pHelper), a GFP control vector, and your choice of 5 different expression vectors. For more information, click here.