Protein Nitration Assays and Reagents

Nitric oxide influences a variety of biological processes including cell proliferation, apoptosis, neurotoxicity and extracellular matrix remodeling. Nitric oxide reacts with superoxide to form peroxynitrite, which in turn nitrates tyrosine residues in proteins. Nitrotyrosine therefore serves as a marker for peroxynitrite action in a variety of disease states and in conditions of cellular damage and oxidative stress.

Our OxiSelect™ Nitrotyrosine Assay Kits provide a simple method to measure the formation of 3-nitrotyrosine in proteins. This assay is available in two formats: a 96-well competitive ELISA and an immunoblotting kit.

1. Wray, W. et al. (2012). Acute Reversal of Endothelial Dysfunction in the Elderly After Antioxidant Consumption. Hypertension. 59:818-824 (#STA-305).
2. Dong, Y.F. et al. (2011). Beneficial Effects of Combination of Valsartan and Amlodipine on Salt-Induced Brain Injury in Hypertensive Rats. J. Pharmacol. Exp. Ther. 339:358-366 (#STA-305).
3. Andreazza, A.C. et al. (2010). Mitochondrial Complex I Activity and Oxidative Damage to Mitochondrial Proteins in the Prefrontal Cortex of Patients with Bipolar Disorder. Arch. Gen. Psychiatry 67:360-368 (#STA-305).
4. Drel, V.R. et al. (2009). Poly(adenosine 5'-diphosphate-ribose) Polymerase Inhibition Counteracts Multiple Manifestations of Experimental Type 1 Diabetic Nephropathy. Endocrinology 150:5273-5283 (#STA-305).
5. Drel, V.R. et al. (2009). New Therapeutic and Biomarker Discovery for Peripheral Diabetic Neuropathy: PARP Inhibitor, Nitrotyrosine, and Tumor Necrosis Factor-Alpha. Endocrinology 10.1210/en.2009-1342 (#STA-305).
6. Cheah, F.-C. et al. (2009). Airway Inflammatory Cell Responses to Intra-Amniotic Lipopolysaccharide in a Sheep Model of Chorioamnionitis. Am. J. Physiol. Lung Cell Mol. Physiol. 296:L384-L393 (#STA-305).
7. Li, X. et al. (2008). Lipoamide Protects Retinal Pigment Epithelial Cells from Oxidative Stress and Mitochondrial Dysfunction. Free Radic. Biol. Med. 44(7):1465-1474 (#STA-305).