The OxiSelect™ ROS Assay Kit is a cell-based assay for measuring hydroxyl, peroxyl, and other reactive oxygen species activity within a cell. The assay employs the cell-permeable fluorogenic probe DCFH-DA, which diffuses into cells and is deacetylcated by cellular esterases into the non-fluorescent DCFH (Figure 1). In the presence of ROS, DCFH is rapidly oxidized to highly fluorescent DCF. Fluorescence is read on a standard fluorometric plate reader.

1. Cho, K.A. et al. (2012). IL-17 and IL-22 Enhance Skin Inflammation by Stimulating the Secretion of IL-1ß by Keratinocytes via the ROS-NLRP3-Caspase-1 Pathway. Int. Immunol. 10.1093/intimm/dxr110.
2. Martin, S.A. et al. (2011). Parallel High-Throughput RNA Interference Screens Identify PINK1 as a Potential Therapeutic Target for the Treatment of DNA Mismatch Repair-Deficient Cancers. Cancer Res. 71:1836-1848.
3. Kuznetsov, J.N. et al. (2011). AMPK and Akt Determine Apoptotic Cell Death following Perturbations of One-Carbon Metabolism by Regulating ER Stress in Acute Lymphoblastic Leukemia. Mol. Cancer Ther. 10:437-447.
4. Wei, J. et al. (2011). C-Jun N-terminal Kinase (JNK-1) Confers Protection Against Brief but not Extended Ischemia during Acute Myocardial Infarction. J. Biol. Chem. 286:13995-14006.
5. Titos, E. et al. (2011). Resolvin D1 and its Precursor Docosahexaenoic Acid Promote Resolution of Adipose Tissue Inflammation by Eliciting Macrophage Polarization toward an M2-Like Phenotype. J. Immunol. 187:5408-5418.
6. Zhang, J. et al. (2011). 2-Deoxy-D-Glucose Attenuates Isoflurane-Induced Cytotoxicity in an In Vitro Cell Culture Model of H4 Human Neuroglioma Cells. Anesth. Analg. 113:1468-1475.
7. Zhang, Y. et al. (2010). The mitochondrial pathway of anesthetic isofluorane-induced apoptosis. J. Biol. Chem. 285:4025-4037.
8. Batova, A. et al. (2010). The Synthetic Caged Garcinia Xanthone Cluvenone Induces Cell Stress and Apoptosis and Has Immune Modulatory Activity. Mol. Cancer Ther. 9:2869-2878.
9. Tanaka, N. et al. (2010). Cis-dichlorodiammineplatinum Upregulates Angiotensin II Type 1 Receptors through Reactive Oxygen Species Generation and Enhances VEGF Production in Bladder Cancer. Mol. Cancer Ther. 9:2982-2992.