Replication Competent Adenovirus Assay

Adenoviral Expression

More accurate than traditional plaque-forming unit assays
Faster results: 2.5 days vs. 10 days
No agar overlay steps

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Product Details
Manuals/MSDS

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Product Name	Catalog Number	Size	Detection	Qty.	Price
Rapid RCA Assay Kit	VPK-111	30 assays	Immunocytochemistry		Select Country
Rapid RCA Assay Kit	VPK-111-5	5 x 30 assays	Immunocytochemistry		Select Country

Overview

Traditionally RCA (replication competent adenovirus) is measured in permissive cells by a plaque-forming unit (PFU) assay which takes 10-14 days. Our Rapid RCA Assay Kit uses an immunocytochemistry staining protocol that requires only a two day incubation.

Figures

Figure 1: Assay Principle for the Rapid RCA Assay.

Figure 1

Figure 2: Staining Results from the Rapid RCA Assay. Serial 10-fold dilutions of Ad5 (2 x 10^12 viral particles/mL) were used to infect A549 cells in a 6-well plate for 48 hours. Anti-Hexon immunostaining was performed according to the assay protocol.

Figure 2

Product Name	Catalog Number	Manual / Data Sheet	MSDS
Rapid RCA Assay Kit	VPK-111	Download	Download
Rapid RCA Assay Kit	VPK-111-5	Download	Download

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