Retroviral Packaging Cells for iPS Cell Generation

iPS Cell Generation

Higher retroviral yields: average titer 10^7 infectious units/mL with transient transfection
Longer stability: up to 4 months in the presence of drug selection
Three versions: ecotropic (Plat-E), amphotropic (Plat-A), and pantropic (Plat-GP)

NOTE: Platinum Retroviral Packaging Cells are available for sale to academic, government and non-profit research laboratories. All other purchasers require a commercial license for all fields including research use. Please contact our Business Development department for license information.

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Product Name	Catalog Number	Size	Detection	Price
Platinum-A Retroviral Packaging Cell Line, Amphotropic	RV-102	1 vial	N/A	Select Country
Platinum-E Retroviral Packaging Cell Line, Ecotropic	RV-101	1 vial	N/A	Select Country
Platinum-GP Retroviral Packaging Cell Line, Pantropic	RV-103	1 vial	N/A	Select Country

Overview

Conventional cells used for retrovirus packaging, such as those based on NIH3T3 cells, have limited stability and produce relatively low yields of retrovirus, mainly due to the poor expression of retroviral structure proteins (gag, pol and env) in the cells.

The Platinum Retroviral Packaging Cell Lines are based on the 293T cell line. They exhibit longer stability and produce higher yields of retroviral structure proteins. Plat-A and Plat-E cells contain gag, pol and env genes, allowing retroviral packaging with a single plasmid transfection.

Suitability of Retroviral Packaging Cell Lines by Host
	Plat-A Cells (Amphotropic)	Plat-E Cells (Ecotropic)	Plat-GP Cells (Pantropic*)
Human	+++	N.S.	+++
Mouse	+++	+++	+++
Rat	+++	+++	+++
Monkey	+++	N.S.	+++
Cat	+++	N.S.	+++
Dog	+++	N.S.	+++
Hamster	+	N.S.	+++
Bird	N.S.	N.S.	+++
Fish	N.S.	N.S.	+++
Frog	N.S.	N.S.	+++
Insect	N.S.	N.S.	+++
Mollusk	N.S.	N.S.	+++
*Retrovirus must be packaged with a pantropic envelope protein such as VSVG N.S. = Not Suitable

Selection Guide for Platinum Retroviral Packaging Cells by Target Host.

Figures

Figure 1: High Retroviral Yields with Plat-E cells. NIH3T3 cells and mouse ProB Ba/F3 cells

Figure 1

Figure 2: High Retroviral Yields with Plat-E cells. NIH3T3 cells were infected with GFP retrovirus supernatant produced in Plat-E cells after transfection with pMX-GFP.

Figure 2

Product Name	Catalog Number	Manual / Data Sheet	MSDS
Platinum-A Retroviral Packaging Cell Line, Amphotropic	RV-102	Download	Download
Platinum-E Retroviral Packaging Cell Line, Ecotropic	RV-101	Download	Download
Platinum-GP Retroviral Packaging Cell Line, Pantropic	RV-103	Download	Download

Recent Product Citations

Togami, H. et al. (2013).Comprehensive In Vitro Analysis of Simian Retrovirus Type 4 Susceptibility to Antiretroviral Agents. J. Virol. 87:4322-4329 (#RV-103).
Nowakowski, T. et al. (2013). MicroRNA-92b Regulates the Development of Intermediate Cortical Progenitors in Embryonic Mouse Brain. PNAS. 110:7056-7061 (#RV-103).
Zhong, S. et al. (2013). T-Cell Receptor Affinity and Avidity Defines Antitumor Response and Autoimmunity in T-Cell Immunotherapy. PNAS. 110:6973-6978 (#RV-101).
Sievert, A. et al. (2013). Paradoxical Activation and RAF Inhibitor Resistance of BRAF Protein Kinase Fusions Characterizing Pediatric Astrocytomas. PNAS. 110:5957-5962 (#RV-101).
Jadav, R. et al. (2013). Inositol Pyrophosphate Synthesis by Inositol Hexakisphosphate Kinase 1 Is Required for Homologous Recombination Repair. J. Biol. Chem.288:3312-3321 (#RV-101).
Kim, G. et al. (2013). A Novel Role for IL-27 in Mediating the Survival of Activated Mouse CD4 T Lymphocytes. J. Immunol.190:1510-1518 (#RV-101).
Chang, J. et al. (2013). MyD88 is Essential to Sustain mTOR Activation Necessary to Promote T Helper 17 Cell Proliferation by Linking IL-1 and IL-23 Signaling. PNAS.110:2270-2275 (#RV-101).
Hrdlickova, R. et al. (2012).Alternatively Spliced Telomerase Reverse Transcriptase Variants Lacking Telomerase Activity Stimulate Cell Proliferation. Mol. Cell. Biol. 32:4283-4296 (#RV-101 & #RV-102).
Koso, H. et al. (2012). PNAS Plus: Transposon Mutagenesis Identifies Genes that Transform Neural Stem Cells into Glioma-Initiating Cells. PNAS. 10.1073/pnas.1215899109 (#RV-101).
Pagnon, J. et al. (2012). Identification and Functional Characterization of Protein Kinase A Phosphorylation Sites in the Major Lipolytic Protein, Adipose Triglyceride Lipase. Endocrinology. 153:4278-4289 (#RV-101).
Seo, W. et al. (2012). Runx1–Cbfβ Facilitates Early B Lymphocyte Development by Regulating Expression of Ebf1. J. Exp. Med. 209: 1255-1262 (#RV-101).
Anantpadma, M and Vrati S.(2012) siRNA-Mediated Suppression of Japanese Encephalitis Virus Replication in Cultured Cells and Mice. J.Antimicrob Chemother. 67:444-451 ( #RV-101).
Braunstein, M. et al. (2010). HEBALt Enhances the T-cell Potential of Fetal Myeloid-biased Precursors. Int. Immunol. 22:963-972 (#RV-101).
Satpathy, S. et al. (2010). Inhibition of Terminal Differentiation of B Cells Mediated by CD27 and CD40 Involves Signaling through JNK. J. Immunol. 185:6499-6507 (#RV-101).
Lee, J-H. et al. (2010). Epigallocatechin-3-Gallate Inhibits Osteoclastogenesis by Down-Regulating C-Fos Expression and Suppressing the Nuclear Factor k-B Signal. Mol Pharmacol. 77:17-25 (#RV-101).
Li, W. et al. (2009). Rb/Cdk2/Cdk4 Triple Mutant Mice Elicit an Alternative Mechanism for Regulation of the G1/S Transition. PNAS 106:486-491 (#RV-101).
Huse, J.T. et al. (2009). The PTEN-Regulating MicroRNA Mir-26a is Amplified in High-grade Glioma and Facilitates Gliomagenesis in Vivo. Genes & Dev. 23:1327-1337 (#RV-101).
Hinrichs, C.S. et al. (2009). Adoptively Transferred Effector Cells Derived from Naive Rather Than Central Memory CD8+ Cells Mediate Superior Antitumor Immunity. PNAS 106:17469-17474 (#RV-101).
Rice, K.L. et al. (2009). Comprehensive Genetic Screens Identify a Role for PLZR-RARalpha as a Positive Regulator of Cell Proliferation via Direct Regulation of c-myc. Blood 114:5499-5511 (#RV-101).
Cui, G. et al. (2009). Berberine Differentially Modulates the Activities of ERK, p38 MAPK, and JNK to Suppress Th17 and Th1 T Cell Differentiation in Type 1 Diabetic Mice. J. Biol. Chem. 284:28420-28429 (#RV-101).
Wright, L.N. et al. (2012). Modeling the Transcriptional Consequences of Epidermal Growth Factor Receptor Ablation in Ras-Initiated Squamous Cancer. Clin. Cancer Res. 18:170-183. (#RV-102).
Qiao, Y. et al. (2011). FOXQ1 Regulates Epithelial-Mesenchymal Transition in Human Cancers. Cancer Res. 71:3076-3086 (#RV-102).
Oda, Y. et al. (2010). Induction of Pluripotent Stem Cells from Human Third Molar Mesenchymal Stromal Cells. J. Biol. Chem. 285:29270-29278 (#RV-102).
Hudecek, M. et al. (2010). The B-cell Tumor-Associated Antigen ROR1 can be Targeted with T-Cells Modified to Express a ROR1-specific Chimeric Antigen Receptor. Blood 116:4532-4541 (#RV-102).
Wu, Y. et al. (2009). Improved Coinfection with Amphotropic Pseudotyped Retroviral Vectors. J. Biochem. Biotechnol. 10.1155/2009/901079 (#RV-102).
Cavnar, P.J. et al. (2011). Hax1 Regulates Neutrophil Adhesion and Motility through RhoA. J. Cell Biol. 10.1083/jcb.201010143 (#RV-103).

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