Induced Pluripotent Stem Cells (iPSC) were originally produced by co-transducing 4 retroviruses, each containing one of the following genes: Oct 3/4, Sox2, Klf4 and c-Myc. Later studies included the use of Lin-28 and/or NANOG.
Cell Biolabs is proud to provide the expression plasmids containing these 6 genes in the pMX backbone, which was used in the pioneering studies of Dr. Yamanaka. In addition to the individual constructs containing either human or mouse sequences, we offer them in complete sets of the original 4 vectors as well as all 6 vectors at a reduced package price.
For higher iPS reprogramming efficiencies, consider one of our polycistronic plasmids. These constructs contain a fusion of the 4 original genes plus a GFP reporter gene.