The SNL 76/7 cell line, established by Dr. Allan Bradley (1), is clonally derived from a mouse fibroblast STO cell line transformed with neomycin resistance and murineLIF genes. SNL can be used as a feeder cell for ES cell growth, and it also has been recently used in mouse or human iPS culture (2, 3).
1. McMahon, A.P. and Bradley, A. (1990) Cell 62:1073–1085.
2. Okita, K; Ichisaka, T; Yamanaka, S. (2007) Nature 448:313–317.
3. Takahashi K, Okita K, Nakagawa M, Yamanaka S. (2007) Nat Protoc 2:3081-9.