Our StemTAG™ 96-Well Stem Cell Colony Formation Assay provides a high-throughput method to quantify ES cells in just 7-10 days, and no manual cell counting is required. Once colonies are formed, they may be analyzed in three different ways:
1. Lyse cells, then quantify in a fluorescence plate reader using dye included in the kit.
2. Lyse cells, then quantify alkaline phosphatase activity using reagents provided.
3. Recover colonies from matrix for further culture or analysis.