Our OxiSelect™ Cellular UV-Induced DNA Damage ELISA Kit measures the formation of 6-4PP in intact cells in a 96-well ELISA plate-based format. Cells are first seeded in a 96-well tissue culture plate. Wells are then UV irradiated to induce DNA damage. After fixation and denaturation, cells containing the DNA lesions are probed with an anti-6-4PP antibody, followed by an HRP conjugated secondary antibody.
DNA Damage Induced by UV Light. Calf thymus DNA at 0.2 mg/mL was exposed to UV light inside a cell culture hood for the time indicated. The CPD levels in 40 ng denatured DNA samples were determined as described in the Assay Protocol for CPD.
DNA Damage Induced by UV Light. Calf thymus DNA at 0.2 mg/mL was exposed to UV light inside a cell culture hood for the time indicated. The 6-4PP levels in 40 ng denatured DNA samples were determined as described in the Assay Protocol for 6-4PP.
DNA Damage Induced by UV Light in Hela Cells. HeLa cells were seeded at 20K/well overnight, then exposed to light under a germicidal lamp for 30 minutes. Immunofluorescence staining of CPD damage was determined as described in the Assay Protocol for #STA-327.
DNA Damage Induced by UV Light in NIH3T3 Cells. NIH3T3 cells were seeded at 20K/well overnight, then exposed to light under a germicidal lamp for 30 minutes. Relative 6-4PP damage was determined as described in the Assay Protocol for #STA-328.
