AAV-DJ/8 CMV Expression System

  • Complete Expression System contains packaging plasmids, expression vector and GFP control vector
  • pHelper plasmid contains required E2A, E4, and VA RNA adenoviral genes; eliminates the need for a helper adenovirus
  • AAV-DJ system provides a hybrid capsid created from 8 different AAV serotypes to provide substantially higher infectivity rates across a broad range of tissues
  • AAV-DJ/8 is a mutant of AAV-DJ designed to provide increased uptake in brain tissue, closely mimicking AAV-8 and AAV-9
  • Cloning capacity of expression vector = 3 kb

 

NOTE: AAV-DJ/8 Helper Free Systems are available for sale to academic, government and non-profit research laboratories. All other purchasers require a commercial license for all fields including research use. Please contact our Business Development department for license information.

 

Frequently Asked Questions about AAV Expression and Packaging

General FAQs about using AAV

General FAQs about Viral Gene Delivery

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AAV-DJ/8 Helper Free Expression System
Catalog Number
VPK-410-DJ-8
Size
1 kit
Detection
N/A
Manual/Data Sheet Download
SDS Download
Map Download
Sequence Download
Price
$1,025.00
Product Details

The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1).

AAV Helper Free Complete Expression Systems are available for native serotypes 1 through 6, as well as the novel AAV-DJ and AAV-DJ/8. The AAV-DJ system provides a hybrid capsid created by DNA shuffling technology combining 8 different native serotypes: AAV-2, AAV-4, AAV-5, AAV-8, AAV-9, avian AAV, bovine AAV, and caprine AAV. The result is a highly infectious vector that can transduce a wide variety of cells and tissues at significantly higher rates than AAV-21.

AAV-DJ/8 was created by making a point mutation in the heparin binding domain of AAV-DJ. Recombinant AAV produced from the AAV-DJ/8 system closely resembles AAV-8 and AAV-9 in its ability to infect heart and brain tissues.

1. Grimm, D. et al. (2008). J. Virol. 82: 5887-5911.

Production and Transduction of Recombinant AAV. AAV2-GFP was produced by cotransfection of 293AAV cells (Catalog #AAV-100) with pAAV-GFP, pAAV-RC2, and pHelper plasmids. Upon harvesting viral supernatant, 293AD cells (Catalog #AD-100) were infected with AAV2-GFP for 48 hours.

Generation of AAV-DJ through Capsid DNA Family Shuffling.