Adenovirus Transduction

Adenovirus Transduction
  • Increases transduction efficiency of adenovirus up to 12-fold in cells expressing little or no CAR (coxsackievirus-adenovirus receptor)
  • May also increase efficiency in some CAR-expressing cells
  • Requires only a short incubation prior to infection

 

Frequently Asked Questions about Adenovirus Purification

General FAQs about using Adenovirus

General FAQs about Viral Gene Delivery

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ViraDuctin™ Adenovirus Transduction Reagent
Catalog Number
AD-200
Size
10 transductions
Detection
N/A
Manual/Data Sheet Download
SDS Download
Price
$325.00
ViraDuctin™ Adenovirus Transduction Reagent
Catalog Number
AD-201
Size
50 transductions
Detection
N/A
Manual/Data Sheet Download
SDS Download
Price
$815.00
Product Details

Adenoviral infection of most cells is mediated largely by the coxsackievirus-adenovirus receptor (CAR). In many cells, however, this receptor is either absent or present at extremely low levels, reducing the efficiency of adenovirus uptake.

ViraDuctin™ Adenovirus Transduction Reagent is designed specifically to increase the efficiency of adenoviral transduction without regard to the level of CAR expression on the surface of the target cells.

*Note: The number of transductions per vial is based on the use of 6-well plates or 35 mm culture dishes. However, this product may also be used with 96-well, 24-well or 12-well plates, as well as 60 mm or 100 mm dishes. See product manual for details.

Enhanced Transduction using the ViraDuctin™ Adenovirus Transduction Reagent. Infection of NIH3T3 cells with recombinant ß-gal Recombinant Adenovirus (#ADV-002). X-gal staining under microscope.

Enhanced Transduction using the ViraDuctin™ Adenovirus Transduction Reagent. Infection of NIH3T3 cells with recombinant ß-gal Recombinant Adenovirus (#ADV-002). Scoring of infection with ViraDuctin™ reagent as a percentage of infection compared to control.

Recent Product Citations
  1. Liu, X. et al. (2020). TRIM62 knockout protects against cerebral ischemic injury in mice by suppressing NLRP3-regulated neuroinflammation. Biochem Biophys Res Commun. 529(2):140-147. doi: 10.1016/j.bbrc.2020.06.014.
  2. Dong, W. et al. (2018). Retinoic acid receptor α expression exerts an anti-apoptosis effect on PC12 cells following oxygen-glucose deprivation. Exp Ther Med. 16(4):3525-3533. doi: 10.3892/etm.2018.6639.
  3. Haidari, M. et al. (2014). Disruption of endothelial adherens junctions by high glucose is mediated by protein kinase C-β-dependent vascular endothelial cadherin tyrosine phosphorylation. Cardiovasc Diabetol. 13:112.
  4. Ackerman, W. et al. (2008). Nuclear Factor-kappa B regulates inducible prostaglandin E synthase expression in human amnion mesenchymal cells. Biol. Reprod. 78:68-76.
  5. Monick, M. et al. (2008). Constitutive ERK MAPK activity regulates macrophage ATP production and mitochondrial integrity. J. Immunol. 180:7485-7496.