Alkaline Phosphatase Activity Assay Combo Kits (96-well and ICC)

Alkaline Phosphatase Activity Assay Combo Kits (96-well and ICC)
  • Measure the ubiquitous alkaline phosphatase marker in embryonic stem cells and embryonic germ cells
  • Provides two assay methods: detect by ICC staining and quantify using 96-well colorimetric or fluorometric detection

 

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StemTAG™ Alkaline Phosphatase Staining and Activity Assay Kit
Catalog Number
CBA-302
Size
2 x 100 assays
Detection
ICC / Colorimetric
Manual/Data Sheet Download
SDS Download
Price
$530.00
StemTAG™ Alkaline Phosphatase Staining and Activity Assay Kit
Catalog Number
CBA-308
Size
2 x 100 assays
Detection
ICC / Fluorometric
Manual/Data Sheet Download
SDS Download
Price
$585.00
Product Details

Alkaline Phosphatase (AP) is a widely used marker for both mouse and human embryonic stem cells (ES) and embryonic germ cells (EG). Our StemTAG™ Alkaline Phosphatase kits provide an efficient system for monitoring cell differentiation or undifferentiation using the AP marker.

The StemTAG™ Alkaline Phosphatase Activity Assay Combo Kits provide reagents for monitoring alkaline phosphatase activity via immunocytochemistry staining as well as in a 96-well plate with either colorimetric or fluorescence detection.

Staining of Undifferentiated and Differentiated ES cells using the StemTAG™ Alkaline Phosphatase Staining Kit. Murine embryonic stem cells (ES-D3) were maintained in an undifferentiated state on gelatin-coated dishes in the presence of LIF. To induce differentiation, LIF was withdrawn over a period of several days; various differentiation events were observed (cells became flattened and enlarged with reduced proliferation). At the end of day 5, cells were stained according to the StemTAG™ Alkaline Phosphatase Staining Kit assay protocol.

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