Arf Activation Assays

Arf Activation Assays
  • Safe non-radioactive assay format
  • Colored agarose beads allow visual check
  • Fast results: 1 hour plus electrophoresis/blotting time
  • Arf1 compatible with human, mouse, and rat samples; Arf6 with human, rat, bovine and canine

 

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Arf1 Activation Assay
Catalog Number
STA-407-1
Size
20 assays
Detection
Immunoblot
Manual/Data Sheet Download
SDS Download
Price
$625.00
Arf6 Activation Assay
Catalog Number
STA-407-6
Size
20 assays
Detection
Immunoblot
Manual/Data Sheet Download
SDS Download
Price
$625.00
Product Details

Our Arf Activation Assays use visible agarose beads to selectively precipitate the active form of Arf1 or Arf 6. The precipitated small GTPase is then detected by Western blot using an Arf1- or Arf6-specific antibody included in the kit.

Small GTPase Activation Assay Principle

Recent Product Citations
  1. Jiang, W. et al. (2020). Apigenin and Ethaverine Hydrochloride Enhance Retinal Vascular Barrier In Vitro and In Vivo. Transl Vis Sci Technol. 9(6):8. doi: 10.1167/tvst.9.6.8 (#STA-407-6).
  2. Kim, S. et al. (2020). Loss of IQSEC3 Disrupts GABAergic Synapse Maintenance and Decreases Somatostatin Expression in the Hippocampus. Cell Rep. 30(6):1995-2005.e5. doi: 10.1016/j.celrep.2020.01.053 (#STA-407-6).
  3. Musi, E. et al. (2019). Tris DBA palladium is an orally available inhibitor of GNAQ mutant uveal melanoma in vivo. Oncotarget. 10(43):4424-4436. doi: 10.18632/oncotarget.27040 (#STA-407-6).
  4. Gilbert, C.E. et al. (2018). Commonly used trafficking blocks disrupt ARF1 activation and the localization and function of specific Golgi proteins. Mol Biol Cell. 29(8):937-947. doi: 10.1091/mbc.E17-11-0622 (#STA-407-1).
  5. Vivien Chiu, W.Y. et al. (2017). GULP1/CED-6 ameliorates amyloid-β toxicity in a Drosophila model of Alzheimer's disease. Oncotarget. 8(59):99274-99283. doi: 10.18632/oncotarget.20062 (#STA-407-6).
  6. Zhu, W. et al. (2017). Small GTPase ARF6 controls VEGFR2 trafficking and signaling in diabetic retinopathy. J Clin Invest. 127(12):4569-4582. doi: 10.1172/JCI91770 (#STA-407-6).
  7. Casey, C.A. et al. (2016). Study of ethanol-induced Golgi disorganization reveals the potential mechanism of alcohol-impaired N-glycosylation. Alcoholism: Clin. and Exp. Res. doi:10.1111/acer-13247 (#STA-407-1).
  8. Fischer. A. et al (2016). AKT-dependent phosphorylation of the SAM domain induces oligomerization and activation of the scaffold protein CNK1. Biochimica et Biophysica Acta - Mol. Cell Res. 1864:89-100 (#STA-407-1).
  9. Matsumoto, Y. et al. (2016). Inhibition of epitehlial-mesenchymal transition by cetuximab via the EGFR-GEP100-Arf6-AMAP1 pathway in head and neck cancer. Head Neck doi:10.1002/hed.24626 (#STA-407-6).
  10. Dutta, D. & Donaldson, J. G.  (2015). Sorting of clathrin‐independent cargo proteins depends on Rab35 delivered by clathrin‐mediated endocytosis. Traffic. doi: 10.1111/tra.12302 (#STA-407-6). 
  11. Loskutov, Y. V. et al. (2014). NEDD9/Arf6-dependent endocytic trafficking of matrix metalloproteinase 14: a novel mechanism for blocking mesenchymal cell invasion and metastasis of breast cancer. Oncogene. doi: 10.1038/onc.2014.297 (#STA-407-1).
  12. Cheung, H. N. et al. (2014). FE65 interacts with ADP-ribosylation factor 6 to promote neurite outgrowth. FASEB J.  28:337-349 (#STA-407-6).
  13. Grossmann, A. Et al. (2013). The Small GTPase ARF6 Stimulates β-Catenin Transcriptional Activity During WNT5A-Mediated Melanoma Invasion and Metastasis. Sci. Signal. 6:ra14 (#STA-407-6).