Arf1 Activation Assay

Arf Activation Assays
  • Safe non-radioactive assay format
  • Colored agarose beads allow visual check
  • Fast results: 1 hour plus electrophoresis/blotting time
  • Arf1 compatible with human, mouse, and rat samples

 

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Arf1 Activation Assay
Catalog Number
STA-407-1
Size
20 assays
Detection
Immunoblot
Manual/Data Sheet Download
SDS Download
Price
$640.00
Product Details

Our Arf1 Activation Assay use visible agarose beads to selectively precipitate the active form of Arf1. The precipitated small GTPase is then detected by Western blot using an Arf1-specific antibody included in the kit.

Small GTPase Activation Assay Principle

Recent Product Citations
  1. Gilbert, C.E. et al. (2018). Commonly used trafficking blocks disrupt ARF1 activation and the localization and function of specific Golgi proteins. Mol Biol Cell. 29(8):937-947. doi: 10.1091/mbc.E17-11-0622.
  2. Casey, C.A. et al. (2016). Study of ethanol-induced Golgi disorganization reveals the potential mechanism of alcohol-impaired N-glycosylation. Alcoholism: Clin. and Exp. Res. doi:10.1111/acer-13247.
  3. Fischer. A. et al (2016). AKT-dependent phosphorylation of the SAM domain induces oligomerization and activation of the scaffold protein CNK1. Biochimica et Biophysica Acta - Mol. Cell Res. 1864:89-100.
  4. Loskutov, Y. V. et al. (2014). NEDD9/Arf6-dependent endocytic trafficking of matrix metalloproteinase 14: a novel mechanism for blocking mesenchymal cell invasion and metastasis of breast cancer. Oncogene. doi: 10.1038/onc.2014.297.