The Cell Biolabs Bacterial Protein Extraction Reagents contain a gentle, nonionic detergent formulation which quickly extracts functional, recombinant protein from E. coli without mechanical disruption.
Extraction of His-RFP fusion protein. His-RFP expression in BL21 was induced with 1 mM IPTG at 37ºC for 4 hrs. Cells were pelleted (left), then resuspended in Bacterial Protein Extraction Reagent for 15 minutes, according to the assay protocol. The supernatant (right) containing the extracted His-RFP was collected from the insoluble post-extraction pellet (center).
Comparison of Sonication with Bacterial Protein Extraction Reagent. Pelleted His-RFP cells were sonicated in PBS/Triton X-100 or resuspended in Bacterial Protein Extraction Reagent for 15 minutes. Lysates were then collected and tested for RFP fluorescence on a 96-well fluorometer.