Bacterial Protein Extraction Reagents

  • Created for small-scale or large-scale protein extraction
  • Compatible with 6xHis and GST affinity purification systems
  • Designed specifically for mild extraction of functional, recombinant proteins from E. coli without mechanical disruption 
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5X Bacterial Protein Extraction Reagent (Tris)
Catalog Number
AKR-180
Size
50 mL
Detection
N/A
Manual/Data Sheet Download
SDS Download
Price
$195.00
5X Bacterial Protein Extraction Reagent (Phosphate)
Catalog Number
AKR-181
Size
50 mL
Detection
N/A
Manual/Data Sheet Download
SDS Download
Price
$195.00
Product Details

The Cell Biolabs Bacterial Protein Extraction Reagents contain a gentle, nonionic detergent formulation which quickly extracts functional, recombinant protein from E. coli without mechanical disruption.

Extraction of His-RFP fusion protein. His-RFP expression in BL21 was induced with 1 mM IPTG at 37ºC for 4 hrs. Cells were pelleted (left), then resuspended in Bacterial Protein Extraction Reagent for 15 minutes, according to the assay protocol. The supernatant (right) containing the extracted His-RFP was collected from the insoluble post-extraction pellet (center).

Comparison of Sonication with Bacterial Protein Extraction Reagent. Pelleted His-RFP cells were sonicated in PBS/Triton X-100 or resuspended in Bacterial Protein Extraction Reagent for 15 minutes. Lysates were then collected and tested for RFP fluorescence on a 96-well fluorometer.