BPDE Protein Adduct ELISA

  • For detection and quantitation of benzo(a)pyrene diol epoxide (BPDE) protein adducts
  • BPDE-BSA standard included
  • Suitable for use with protein samples such as purified protein, plasma, serum, or cell lysates

 

Video: Color Development in an ELISA

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OxiSelect™ BPDE Protein Adduct ELISA Kit
Catalog Number
STA-301
Size
96 wells
Detection
Colorimetric
Manual/Data Sheet Download
SDS Download
Price
$635.00
Product Details

BSA standards or protein samples (10 μg/mL) are adsorbed onto a 96-well plate for 2 hrs at 37ºC.  The BPDE-protein adducts present in the sample or standard are probed with an Anti-BPDE-I Antibody, followed by an HRP Conjugated Secondary Antibody.  The BPDE protein adduct content in an unknown sample is determined by comparing with a standard curve that is prepared from predetermined BPDE-BSA standards.

BPDE-BSA Standard Curve.

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