Checkpoint Kinase Activity Assays
Checkpoint Kinases (CHK1 and CHK2) can be activated in response to DNA damage prior to mitosis. Active checkpoint kinases can phosphorylate Cdc25c at Ser-216, which ultimately leads to cell cycle arrest. Mutations in checkpoint kinases can lead to decreased DNA repair and increased susceptibility to cancer. Our Checkpoint Kinase Activity Assays conveniently measure the activity of these important enzymes.