Hexokinases (HK) are important enzyme in the glucose metabolism process. Our Hexokinase Activity Assay Kit is a simple colorimetric assay that quantifies the activity of HK in cell and tissue lysates in a convenient 96-well format. Each kit provides sufficient reagents to perform up to 100 assays, including blanks, standards, and unknown samples. G6PDH levels in an unknown sample are calculated based on a Hexokinase standard curve.