Cell Biolabs’ HIF-1 Alpha Sandwich ELISA Kit is an immunoassay developed for rapid detection of HIF-1 Alpha in any protein sample. HIF-1 Alpha protein is captured by a mouse monoclonal antibody that is attached to the well. HIF-1 Alpha is then detected with a goat polyclonal anti-HIF-1 Alpha antibody followed by an HRP conjugated secondary antibody. Each kit provides sufficient reagents to perform up to a total of 96 assays, and can detect HIF-1 Alpha from human, mouse, or rat.
Ohyama, A. et al. (2016). Establishment and characterization of a clear cell carcinoma cell line, designated NOCC, derived from human ovary. Human Cell. doi:10.1007/s13577-016-0142-x.