Homocysteine is an amino acid intermediate that is synthesized from methionine via S-adenosylmethionine (SAM) and S-adenosylhomocysteine (SAH). Elevated levels of homocysteine can indicate a risk for numerous diseases, including vascular and heart disease.
Our Homocysteine ELISA is designed for detection and quantitation of homocysteine in plasma, serum, lysates, or biological fluid samples. This assay is a competitive ELISA where samples and an anti-homocysteine antibody are added to a plate coated with a homocysteine conjugate. The sample and conjugate compete for antibody binding, which generates a reverse curve. Samples with high homocysteine levels will bind to most of the antibody, which gets washed away and results in a low OD, while samples with low homocysteine levels will leave more antibody available to bind to the conjugate, producing a high signal.