Haptoglobin (Hp) is expressed from the Hp gene in adipose tissue, kidney, skin, lung, and hepatocyte cells. It is secreted into blood plasma where it binds to free hemoglobin (Hb) and prevents Hb from reacting oxidatively. The Hb/Hp complex is removed in the spleen by the mononuclear phagocytic system. Mutations in the Hp gene can lead to ahaptoglobinemia and hypohaptoglobinemia and has been linked to several diseases.
Our Human Haptoglobin ELISA Kit is a sandwich ELISA for the detection of human haptoglobin in plasma, serum, lysates, and other biological fluids. Samples or standards are added to an anti-human Hp antibody coated plate followed by the addition of an anti-human Hp antibody. An HRP conjugated secondary antibody is then added for detection and the absorbance is read at 450nm. The level of haptoglobin in samples is determined based on the provided human haptoglobin standard curve.