Hypoxia Assays

Cell hypoxia, or low oxygen condition, is a normal physiological response to certain body stressors such as high altitudes, but it also can be a symptom of pathological conditions and is often used as a marker for tumor cells. In response to hypoxic conditions, the hypoxia-inducible factor 1 transcriptional activator complex (HIF-1) plays a role in activating several hypoxia-responsive genes such as erythropoietin and VEGF. During hypoxia, the alpha subunit of HIF-1 accumulates and translocates from the cytosol to the nucleus, where it dimerizes with the beta subunit and becomes transcriptionally active. It then binds transcriptional coactivators to induce gene expression.