*For quantitation of serum samples, please use our Colorimetric assay.
Myeloperoxidase (MPO) is a heme-based peroxidase enzyme responsible for antimicrobial activity against a wide range of organisms. It has also been found to be correlated with increased oxidative stress and decreased levels of catalase activity.
The OxiSelect™ Myeloperoxidase Chlorination Activity Assay Kit is a quantitative, fluorometric assay for measuring the myeloperoxidase activity within a sample. The MPO enzyme catalyzes the reaction of hydrogen peroxide (H2O2) with chloride ions to create hypochlorous acid (HOCl), which reacts with a fluorogenic probe to produce a bright fluorescence detected in a 96-well plate reader.