Promoterless Adenoviral Expression System

Promoterless Adenoviral Expression System
  • Engineered to produce 100- to 10,000-fold fewer wild type plaques than most other expression systems
  • Generate high-titer virus in about 2-3 weeks compared to 2-3 months with other systems
  • Promoterless shuttle vector allows you to clone the optimal promoter with your gene of interest
  • Cloning capacity of shuttle vector = 7.5 kb

 

Frequently Asked Questions about this product

General FAQs about using Adenovirus

General FAQs about Viral Gene Delivery

Email To BuyerPrint this PageCopy Link
Ordering

Please contact your distributor for pricing.

RAPAd® Universal Adenoviral Expression System
Catalog Number
VPK-250
Size
1 kit
Detection
N/A
Manual/Data Sheet Download
SDS Download
Map Download
Sequence Download
Price
$920.00
Product Details

Making an adenovirus with traditional recombinant methods takes 2-3 months and requires tedious plaque recombination. More recent technologies have shortened this time somewhat, but still produce relatively high amounts of wild type (replication-competent) plaques, levels of which increase with serial amplification.

The RAPAd® Adenoviral Expression Systems produce recombinant adenovirus with substantially reduced wild-type virus, while considerably shortening the production time to 2-3 weeks. Serial amplification of adenovirus produced using the RAPAd® system does not significantly increase replication-competent adenovirus levels. The system uses a backbone vector from which the 5' ITR, packaging signal and E1 sequences have been removed.

The RAPAd® Universal Adenoviral Expression System (# VPK-250) contains a promoterless shuttle vector to allow you to insert the promoter of your choice along with your gene of interest to maximize gene expression.

Recent Product Citations
  1. Hogan, T.B. et al. (2022). Caveolin-1 peptide regulates p53-microRNA-34a feedback in fibrotic lung fibroblasts. iScience. 25(4):104022. doi: 10.1016/j.isci.2022.104022.
  2. Zhang, Z. et al. (2021). DHHC9-mediated GLUT1 S-palmitoylation promotes glioblastoma glycolysis and tumorigenesis. Nat Commun. 12(1):5872. doi: 10.1038/s41467-021-26180-4.
  3. Kuang, H.J. et al. (2017). Hsp27 promotes ABCA1 expression and cholesterol efflux through the PI3K/PKCζ/Sp1 pathway in THP-1 macrophages. Eur. J. Pharmacol. 810:57-62.
  4. Blanco Fernández, M.D. et al. (2017). Comparison of internal process control viruses for detection of food and waterborne viruses. Appl Microbiol Biotechnol. 101(10):4289-4298. doi: 10.1007/s00253-017-8244-2.
  5. Yan, Y. et al. (2016). HIV-1 Vpr increases HCV replication through VprBP in cell culture. Virus Res. doi:10.1016/j.virusres.2016.07.007.
  6. Li, P. et al. (2013).MicroRNA-663 Regulates Human Vascular Smooth Muscle Cell Phenotypic Switch and Vascular Neointimal Formation. Circ Res. 113:1117-1127.