Tumor Cell Isolation

  • Efficiently eliminate normal cells from tumor cell population in heterogeneous solid tumor samples
  • Recover tumor cells for further downstream analysis
  • Potential use for evaluation of tumor stem cells

 

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CytoSelect™ Clonogenic Tumor Cell Isolation Kit
Catalog Number
CBA-155
Size
5 preps
Detection
N/A
Manual/Data Sheet Download
SDS Download
Price
$645.00
CytoSelect™ Clonogenic Tumor Cell Isolation Kit
Catalog Number
CBA-155-5
Size
25 preps
Detection
N/A
Manual/Data Sheet Download
SDS Download
Price
$2,755.00
Product Details

Many solid tumors contain heterogeneous populations of normal and cancerous cells. Separation of these cell populations is key to an accurate assessment of the true genotypic and phenotypic differences between normal and tumor cells.

Our CytoSelect™ Clonogenic Tumor Cell Isolation Kit uses a proprietary semisolid agar medium to facilitate formation of colonies by cells from solid tumors. Colonies are grown in either a 6-well plate or a 35mm culture dish. These colonies are isolated away from single (i.e. normal) cells by size filtration. The viable cells from these colonies can be easily recovered for further analysis.

CytoSelect™ Clonogenic Tumor Cell Isolation Procedure.

Clonogenic Colony Formation, Isolation and Re-plating. A: Clonogenic colony formation (red arrows) and single cells (black arrows) after 7 day incubation. B: Isolation of clonogenic colonies from single cells. C: Re-plated clonogenic colonies after 3 days (no trypsinization). D: Re-plated clonogenic colonies one day after trypsinization.

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