Tumor Cell Isolation

Tumor Cell Isolation
  • Efficiently eliminate normal cells from tumor cell population in heterogeneous solid tumor samples
  • Recover tumor cells for further downstream analysis
  • Potential use for evaluation of tumor stem cells

 

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CytoSelect™ Clonogenic Tumor Cell Isolation Kit
Catalog Number
CBA-155
Size
5 preps
Detection
N/A
Manual/Data Sheet Download
SDS Download
Price
$660.00
CytoSelect™ Clonogenic Tumor Cell Isolation Kit
Catalog Number
CBA-155-5
Size
25 preps
Detection
N/A
Manual/Data Sheet Download
SDS Download
Price
$2,835.00
Product Details

Many solid tumors contain heterogeneous populations of normal and cancerous cells. Separation of these cell populations is key to an accurate assessment of the true genotypic and phenotypic differences between normal and tumor cells.

Our CytoSelect™ Clonogenic Tumor Cell Isolation Kit uses a proprietary semisolid agar medium to facilitate formation of colonies by cells from solid tumors. Colonies are grown in either a 6-well plate or a 35mm culture dish. These colonies are isolated away from single (i.e. normal) cells by size filtration. The viable cells from these colonies can be easily recovered for further analysis.

CytoSelect™ Clonogenic Tumor Cell Isolation Procedure.

Clonogenic Colony Formation, Isolation and Re-plating. A: Clonogenic colony formation (red arrows) and single cells (black arrows) after 7 day incubation. B: Isolation of clonogenic colonies from single cells. C: Re-plated clonogenic colonies after 3 days (no trypsinization). D: Re-plated clonogenic colonies one day after trypsinization.

Recent Product Citations
  1. Hui, T.H. et al. (2020). Detection of the mesenchymal-to-epithelial transition of invasive non-small cell lung cancer cells by their membrane undulation spectra. RSC Adv. doi: 10.1039/D0RA06255C.
  2. Goulielmaki, E. et al. (2018). Pharmacological inactivation of the PI3K p110δ prevents breast tumour progression by targeting cancer cells and macrophages. Cell Death Dis. 9(6):678. doi: 10.1038/s41419-018-0717-4.
  3. Wang, K. et al. (2017). IL-33 blockade suppresses tumor growth of human lung cancer through direct and indirect pathways in a preclinical model. Oncotarget. 8(40):68571-68582. doi: 10.18632/oncotarget.19786.
  4. Liao, C.J. et al. (2017). Isolation of label-free and viable circulating tumour cells (CTCs) from blood samples of cancer patients through a two-step process: negative selection-type immunomagnetic beads and spheroid cell culture-based cell isolation. RSC Adv. 7:29339-29349.
  5. Feng, J.F. (2017). Advances of Single-Cell Sequencing Technique in Tumors. J Int Transl Med. 5(1):8-13. doi:10.11910/2227-6394.2017.05.01.02.
  6. Hu, H. et al. (2017). IL-33 facilitates endocrine resistance of breast cancer by inducing cancer stem cell properties. Biochem Biophys Res Commun. doi: 10.1016/j.bbrc.2017.02.080.
  7. Wang, C. et al. (2016). IL-33 signaling fuels outgrowth and metastasis of human lung cancer. Biochem Biophys Res Commun. doi:10.1016/j.bbrc.2016.09.081.
  8. Zhao, Y. et al. (2016). Single-cell electrical phenotyping enabling the classification of mouse tumor samples. Sci Rep. 6:19487.