Tumor Cell Isolation

Many solid tumors contain heterogeneous populations of normal and cancerous cells. Separation of these cell populations is key to an accurate assessment of the true genotypic and phenotypic differences between normal and tumor cells.

Our CytoSelect™ Clonogenic Tumor Cell Isolation Kit uses a proprietary semisolid agar medium to facilitate formation of colonies by cells from solid tumors. Colonies are grown in either a 6-well plate or a 35mm culture dish. These colonies are isolated away from single (i.e. normal) cells by size filtration. The viable cells from these colonies can be easily recovered for further analysis.

1. Wang, C. et al. (2016). IL-33 signaling fuels outgrowth and metastasis of human lung cancer. Biochem Biophys Res Commun. doi:10.1016/j.bbrc.2016.09.081.
2. Zhao, Y. et al. (2016). Single-cell electrical phenotyping enabling the classification of mouse tumor samples. Sci Rep. 6:19487.