AAV Helper Free Packaging Systems

AAV Helper Free Packaging Systems

AAV Helper Free Packaging Systems contain the following constructs needed to package your recombinant adeno-associated virus along with your own expression vector:

  • pHelper Plasmid
  • Serotype-specific Rep-Cap Plasmid
  • pAAV-GFP Control Plasmid

AAV Helper Free Packaging Systems are available for native serotypes 1 through 6, as well as the novel AAV-DJ and AAV-DJ/8. The AAV-DJ system provides a hybrid capsid created by DNA shuffling technology combining 8 different native serotypes: AAV-2, AAV-4, AAV-5, AAV-8, AAV-9, avian AAV, bovine AAV, and caprine AAV. The result is a highly infectious vector that can transduce a wide variety of cells and tissues at significantly higher rates than AAV-21.

AAV-DJ/8 was created by making a point mutation in the heparin binding domain of AAV-DJ. Recombinant AAV produced from the AAV-DJ/8 system closely resembles AAV-8 and AAV-9 in its ability to infect heart and brain tissues.

1. Grimm, D. et al. (2008). J. Virol. 82: 5887-5911.

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