Selection Guide for Boyden Chamber Cell Migration and Invasion Assays

Assay Type Definition Cell Types Pore Size Insert Coating Formats
Chemotaxis Migration of cells toward a chemoattractant (chemical signal) in the cell's surrounding environment Leukocytes
Neutrophils
3 µm None 24-Well
96-Well
Lymphocytes
Macrophages
Monocytes
5 µm None 24-Well
96-Well
Endothelial Cells
Epithelial Cells
Fibroblasts
Tumor Cells
8 µm None 24-Well
96-Well
Astrocytes
Slow-moving Cells
12 µm None 24-Well
Haptotaxis Migration of cells along a gradient of cellular adhesion sites or extracellular matrix-bound chemoattractants Endothelial Cells
Epithelial Cells
Fibroblasts
8 µm Collagen I (bottom) 24-Well
Fibronectin (bottom) 24-Well
Transmigration Migration of cells through the vascular endothelium toward a chemoattractant Leukocytes 3 µm None 24-Well
Tumor Cells 8 µm None 24-Well
Invasion Movement of cells through the 3D extracellular matrix into neighboring tissues; includes ECM degradation and proteolysis Endothelial Cells
Epithelial Cells
Fibroblasts
Tumor Cells
8 µm ECM Matrix (top) 24-Well
96-Well
Collagen I (top) 24-Well
96-Well
Laminin I (top) 24-Well
96-Well

 

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