Selection Guide for Boyden Chamber Cell Migration and Invasion Assays
| Assay Type | Definition | Cell Types | Pore Size | Insert Coating | Formats |
| Chemotaxis | Migration of cells toward a chemoattractant (chemical signal) in the cell's surrounding environment |
Leukocytes Neutrophils |
3 µm | None |
24-Well 96-Well |
|
Lymphocytes Macrophages Monocytes |
5 µm | None |
24-Well 96-Well |
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|
Endothelial Cells Epithelial Cells Fibroblasts Tumor Cells |
8 µm | None |
24-Well 96-Well |
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|
Astrocytes Slow-moving Cells |
12 µm | None | 24-Well | ||
| Haptotaxis | Migration of cells along a gradient of cellular adhesion sites or extracellular matrix-bound chemoattractants |
Endothelial Cells Epithelial Cells Fibroblasts |
8 µm | Collagen I (bottom) | 24-Well |
| Fibronectin (bottom) | 24-Well | ||||
| Transmigration | Migration of cells through the vascular endothelium toward a chemoattractant | Leukocytes | 3 µm | None | 24-Well |
| Tumor Cells | 8 µm | None | 24-Well | ||
| Invasion | Movement of cells through the 3D extracellular matrix into neighboring tissues; includes ECM degradation and proteolysis |
Endothelial Cells Epithelial Cells Fibroblasts Tumor Cells |
8 µm | ECM Matrix (top) |
24-Well 96-Well |
| Collagen I (top) |
24-Well 96-Well |
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| Laminin I (top) |
24-Well 96-Well |
