Cell Invasion Assays

The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis.

Our CytoSelect™ Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 96-well plates on a fluorescence plate reader, or in 24-well plates on either a standard microplate reader or a fluorescence plate reader. Inserts are precoated on the top of the membrane with your choice of ECM matrix gel (basement membrane), Collagen I, or Laminin I.

1. Zhang, L. et al. (2011). MicroRNA-1258 Suppresses Breast Cancer Brain Metastasis by Targeting Heparanase. Cancer Res. 71:645-654. (#CBA-110)
2. Piccaluga, P.P. et al. (2011). Gene Expression Analysis Uncovers Similarity and Differences Among Burkitt Lymphoma Subtypes. Blood 117:3596-3608.
3. Coon, B. et al. (2010). The Epsin Family of Endocytic Adaptors Promotes Fibrosarcoma Migration and Invasion. J. Biol. Chem. 285:33073-33081(#CBA-110)
4. Hirata, H. et al. (2010). Role of secreted Frizzled-related protein3 in Human Renal Cell Carcinoma. Cancer Res. 70:1896-1905. (#CBA-110)
5. Chen, H. et al. (2009). Extracellular Signal-Regulated Kinase Signaling Pathway Regulates Breast Cancer Cell Migration by Maintaining Slug Expression. Cancer Res. 69:9228-9235 (#CBA-110).
6. Cabello, C. et al. (2009). The Cinnamon-Derived Michael Acceptor Cinnamic Aldehyde Impairs Melanoma Cell Proliferation, Invasiveness, and Tumor Growth. Free Rad. Biol. and Med. 46(2):220-231. (#CBA-110)
7. Zhang, Q. et al. (2009). Nuclear factor-kB Mediated Transforming Growth Factor-ß-Induced Expression of Vimentin is an Independent Predictor of Biochemical Recurrence after Radial Prostatectomy. Clin. Cancer Res. 15:3557-3567. (#CBA-110)
8. He, X. et al. (2007). Knockdown of Polypyrimidine Tract-Binding Protein Suppresses Ovarian Tumor Cell Growth and Invasiveness in Vivo. Oncogene 26:4961-4968 (#CBA-110).
9. Ji, H. et al. (2007). LKB1 Modulates Lung Cancer Differentiation and Metastasis. Nature 448:807-810. (#CBA-110)
10. Phinney, D. et al. (2006). Murine Mesenchymal Stem Cells Transplanted to the Central Nervous System of Neonatal Versus Adult Mice Exhibit Distinct Engraftment Kinetics and Express Receptors that Guide Neuronal Cell Migration. Stem Cells Dev. 15(3) :437-447 (#CBA-110).
11. Beach, J.R. et al. (2011). Myosin II Isoform Switching Mediates Invasiveness after TGF-ß-Induced Epithelial-Mesenchymal Transition. PNAS 108:17991-17996. (#CBA-112). 
12. Eckstein, N. et al. (2009). Hyperactivation of the Insulin-like Growth Factor Receptor I Signaling Pathway is an Essential Event for Cisplatin Resistance of Ovarian Cancer Cells. Cancer Res. 69:2996-3003. (#CBA-112)
13. Lam, K.K.W. et al. (2009). Glycodelin-A as a Modulator of Trophoblast Invasion. Hum. Reprod. 24:2093-2103. (#CBA-112)
14. Thal, D.R. et al. (2008). Expression of Coronin-3 (Coronin-1C) in Diffuse Gliomas is Related to Malignancy. J. Pathol. 214:415-424 (#CBA-112).
15. Neil, J.R. et al. (2008). Cox-2 Inactivates Smad Signaling and Enhances EMT Stimulated by TGFß through a PGE2-Dependent Mechanism. Carcinogenesis 29:2227-2235 (#CBA-112).