Chemotaxis Assays

Chemotaxis describes the movement of cells toward or away from a chemical stimulus in their enviroment. Cell chemotaxis plays a pivotal role in the progression of cancer and other diseases.

CytoSelect™ Cell Migration Assays are ideal for determining the chemotactic properties of cells. The kits utilize polycarbonate membrane inserts in either 24-well or 96-well plates. Membranes are available in 4 pore sizes to accommodate a variety of cell types and sizes:

• 8 µm pore size is suitable for most cell types including epithelial cells, fibroblasts, and cancer cell lines
• 5 µm pore size is ideal for monocytes / macrophages
• 3 µm pore size is best for the smallest cells including neutrophils and other leukocytes
• 12 µm pore size is suitable for astrocytes and other large or slow-moving cells

1. Al-Wadei, M.H. et al. (2013).Gamma-amino Butyric Acid (GABA) Prevents the Induction of Nicotinic Receptor–Regulated Signaling by Chronic Ethanol in Pancreatic Cancer Cells and Normal Duct Epithelia. Cancer Prevention Research. 6:139-148 (#CBA-100).
2. Kiss, J. et al. (2012).Loss of the Oxygen Sensor PHD3 Enhances the Innate Immune Response to Abdominal Sepsis. J. Immunol. 189:1955-1965 (#CBA-102).
3. Al-Wadei, M.H. et al. (2012).Effects of Chronic Nicotine on the Autocrine Regulation of Pancreatic Cancer Cells and Pancreatic Duct Epithelial Cells by Stimulatory and Inhibitory Neurotransmitters. Carcinogenesis. 33:1745-7153 (#CBA-100).
4. Chen, Z. et al. (2012). The Iron Chelators Dp44mT and DFO Inhibit TGF-β-induced Epithelial-Mesenchymal Transition via Up-Regulation of N-Myc Downstream-regulated Gene 1 (NDRG1). J.Biol.Chem. 287:17016-17028(#CBA-100).
5. Rosenblum, S. et al. (2012). Timing of Intra-Arterial Neural Stem Cell Transplantation After Hypoxia–Ischemia Influences Cell Engraftment, Survival, and Differentiation. Stroke. 43:1624-1631(#CBA-106).
6. Jun, H.S. et al. (2012). Glucose-6-Phosphatase-β, Implicated in a Congenital Neutropenia Syndrome, is Essential for Macrophage Energy Homeostasis and Functionality. Blood. 119:4047-4055 (#CBA-105).
7. Tabata, C. et al. (2010). Novel Clinical Role of Angiopoietin-1 in Malignant Pleural Mesothelioma. Eur. Respir. J. 10.1183/09031936.00154009 (#CBA-100).
8. Awasthi, N. et al. (2009). Endothelial Monocyte Activating Polypeptide II Interferes with VEGF-Induced Proangiogenic Signaling. Laboratory Investigation 89(1):38-46 (#CBA-100).
9. Igarashi, J. et al. (2009). Transforming Growth Factor-ß1 Downregulates Caveolin-1 Expression and Enhances Sphingosine 1-Phosphate Signaling in Cultured Vascular Endothelial Cells. Am. J. Physiol. Cell Physiol. 297:C1263-C1274 (#CBA-100).
10. Tabata, C. et al. (2009). All-Trans-Retinoic Acid Inhibits Tumor Growth of Malignant Pleural Mesothelioma in Mice. Eur. Respir. J. 34:1159-1167 (#CBA-100).
11. Awasthi, N. et al. (2008). Downregulation of MMP-2 and -9 by Proteasome Inhibition: A Possible Mechanism to Decrease LEC Migration and Prevent Posterior Capsular Opacification. Invest. Ophthalmol. Vis. Sci. 49:1998-2003 (#CBA-100).
12. Ma, J. et al. (2008). Pancreatic Duodenal Homeobox-1(PDX-1) Functions as a Tumor Suppressor in Gastric Cancer. Carcinogenesis 29:1327-1333 (#CBA-100).
13. Schuller, H. et al. (2008). Gamma-Aminobutyric Acid, a Potential Tumor Suppressor for Small Airway-Derived Lung Adenocarcinoma.Carcinogenesis 29:1979-1985 (#CBA-100).
14. Lukas, T.J. et al. (2008). Susceptibility to Glaucoma: Differential Comparison of the Astrocyte Transcriptome from Glaucomatous African American and Caucasian American Donors. Genome Biology 9:R111 (#CBA-100).
15. Schuller, H. et al. (2007). GABA B Receptor is a Novel drug Target for Pancreatic Cancer. Cancer 112(4):767-768 (#CBA-100).
16. Murtagh, J. et al. (2006). Taxotere-Induced Inhibition of Human Endothelial Cell Migration is a Result of Heat Shock Protein 90 Degradation. Cancer Research 66:8192-8199 (#CBA-100).
17. Phinney, D. et al. (2006). Murine Mesenchymal Stem Cells Transplanted to the Central Nervous System of Neonatal Versus Adult Mice Exhibit Distinct Engraftment Kinetics and Express Receptors that Guide Neuronal Cell Migration. Stem Cells Dev. 15(3):437-447 (#CBA-100).
18. Lu, H. et al. (2006). The Microtubule Binding Drug Laulimalide Inhibits Vascular Endothelial Growth Factor-Induced Human Endothelial Cell Migration and is Synergistic when Combined with Docetaxel (Taxotere). Mol Pharmacol. 69(4):1207-1215 (#CBA-100).
19. Izhak, L. et al. (2010). Predominant Expression of CCL2 at the Tumor Site of Prostate Cancer Patients Directs a Selective Loss of Immunological Tolerance to CCL2 that Could be Amplified in a Beneficial Manner.J. Immunol.184:1092-1101 (#CBA-101).
20. Izhak, L. et al. (2009). A Novel Recombinant Fusion Protein Encoding a 20-Amino Acid Residue of the Third Extracellular (E3) Domain of CCR2 Neutralizes the Biological Activity of CCL2. J. Immunol. 183:732-739 (#CBA-101).
21. Wang, W. et al. (2009). Netrin-1 Increases Proliferation and Migration of Renal Proximal Tubular Epithelial Cells via the UNC5B Receptor.Am. J. Physiol. Renal Physiol. 296:F723-729 (#CBA-101).
22. Reing, J. et al. (2008). Degradation Products of Extracellular Matrix Affect Cell Migration and Proliferation. Tissue Eng. Part A 10.1089/ten.tea.2007.0425 (#CBA-101).
23. Fava, G. et al. (2008). Leptin Enhances Cholangiocarcinoma Cell Growth. Cancer Res. 68:6752-6761 (#CBA-101).
24. Rayner, K. et al. (2008). Extracellular Release of the Atheroprotective Heat Shock Protein 27 is Mediated by Estrogen and Completely Inhibits acLDL Binding to Scavenger Receptor-A. Circ. Res. 103(2):133-141 (#CBA-102).
25. Shynlova, O. et al. (2008). Monocyte Chemoattractant Protein-1 (CCL-2) Integrates Mechanical and Endocrine Signals that Mediate Term and Preterm Labor. J. Immunol. 181:1470-1479 (#CBA-102)
26. Verma, S. et al. (2011). Selenoprotein K Knockout Mice Exhibit Deficient Calcium Flux in Immune Cells and Impaired Immune Responses. J. Immunol. 186:2127-2137. (#CBA-103). 
27. Li, X. et al. (2011). Kaposi's Sarcoma-Associated Herpesvirus-Encoded Latency-Associated Nuclear Antigen Reduces Interleukin-8 Expression in Endothelial Cells and Impairs Neutrophil Chemotaxis by Degrading Nuclear p65. J. Virol. 85:8606-8615. (#CBA-104). 
28. Chatterjee, S. et al. (2009). Site-Specific Carboxypeptidase B1 Tyrosine Nitration and Pathophysiological Implications following its Physical Association with Nitric Oxide Synthase-3 in Experimental Sepsis. J. Immunol. 183:4055-4066 (#CBA-104).
29. Sarkar, D.K. et al. (2011). Transplantation of ß-Endorphin Neurons into the Hypothalamus Promotes Immune Function and Restricts the Growth and Metastasis of Mammary Carcinoma. Cancer Res. 71:6282-6291. (#CBA-105). 
30. Christophi, G. et al. (2008). Modulation of Macrophage Infiltration and Inflammatory Activity by the Phosphatase SHP-1 in Virus-Induced Demyelinating Disease. J. Virol. 83:522-539 (#CBA-105).
31. Caglayan, E. et al. (2008). Differential Roles of Cardiomyocyte and Macrophage Peroxisome Proliferator-Activated Receptor Gamma in Cardiac fibrosis. Diabetes 57:2470-2479 (#CBA-105).
32. Aftab, B.T. et al. (2011). Itraconazole Inhibits Angiogenesis and Tumor Growth in Non-Small Cell Lung Cancer. Cancer Res. 71:6764-6772. (#CBA-106). 
33. Andres, R.H. et al. (2011). The CCR2/CCL2 Interaction Mediates the Transendothelial Recruitment of Intravascularly Delivered Neural Stem Cells to the Ischemic Brain. Stroke 42:2923-2931 (#CBA-106).
34. Saher, H. et al. (2010). Red wine Consumption Improves in Vitro Migration of Endothelial Progenitor Cells in Young, Healthy Individuals. Am. J. Clinical Nutrition. 92:161-169 (#CBA-106).
35. Zhao, L. et al. (2008). CD44 Expressed on Both Bone Marrow-Derived and Non-Bone Marrow-Derived Cells Promotes Atherogenesis in ApoE-Deficient Mice. Arterioscler. Thromb. Vasc. Biol. 28:1283-1289 (#CBA-106).
36. Beattie, A.J. et al. (2008). Chemoattraction of Progenitor Cells by Remodeling Extracellular Matrix Scaffolds. Tissue Eng. Part A 10.1089/ten.tea.2008.0162 (#CBA-106).