Cancer cell transmigration, particularly extravasation, is an important step in cancer metastasis. It is the final step in a cascade of interactions between cells and the endothelium.
CytoSelect™ Cell Transmigration Assays provide a robust system for the quantitative determination of transmigrations and interactions between endothelium and cancer cells. Migratory cells may be quantified on a fluorescence plate reader. Kits use 24-well plates with membrane inserts:
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The CytoSelect™ Leukocyte Transmigration Assay uses inserts with a 3 µm pore size
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The CytoSelect™ Tumor Transendothelial Migration Assay uses an 8 µm pore size to accommodate larger tumor cells
