Alkaline Phosphatase (AP) is a widely used marker for both mouse and human embryonic stem cells (ES) and embryonic germ cells (EG). Our StemTAG™ Alkaline Phosphatase kits provide an efficient system for monitoring cell differentiation or undifferentiation using the AP marker.
The StemTAG™ Alkaline Phosphatase Complete Kit provides reagents for monitoring alkaline phosphatase activity via both immunocytochemistry staining and a quantitative colorimetric assay, with sufficient reagents for 100 assays of each method. The ICC and colorimetric kits are also sold separately.
Figure 1: Staining of Undifferentiated and Differentiated ES cells using the StemTAG™ Alkaline Phosphatase Staining Kit. Murine embryonic stem cells (ES-D3) were maintained in an undifferentiated state on gelatin-coated dishes in the presence of LIF. To induce differentiation, LIF was withdrawn over a period of several days; various differentiation events were observed (cells became flattened and enlarged with reduced proliferation). At the end of day 5, cells were stained according to the StemTAG™ Alkaline Phosphatase Staining Kit assay protocol.