Alkaline Phosphatase Detection

Alkaline Phosphatase (AP) is a widely used marker for both mouse and human embryonic stem cells (ES) and embryonic germ cells (EG). Our StemTAG™ Alkaline Phosphatase kits provide an efficient system for monitoring cell differentiation or undifferentiation using the AP marker.

The StemTAG™ Alkaline Phosphatase Complete Kit provides reagents for monitoring alkaline phosphatase activity via both immunocytochemistry staining and a quantitative colorimetric assay, with sufficient reagents for 100 assays of each method. The ICC and colorimetric kits are also sold separately.

1. Lee, J. el al. (2010). Ultraviolet A Regulates Adipogenic Differentiation of Human Adipose Tissue-derived Mesenchymal Stem Cells via Up-Regulation of Kruppel-like Factor 2. J. Biol. Chem. 285:32647-32656 (#CBA-300).
2. Izadyar, F. et al. (2008). Generation of multipotent cell lines from a distinct population of male germ line stem cells. Reproduction 135:771-784 (#CBA-300).
3. Moussalli, M.J. et al. (2011). Mechanistic Contribution of Ubiquitous 15-Lipoxygenase-1 Expression Loss in Cancer Cells to Terminal Cell Differentiation Evasion. Cancer Prevention Research 4:1961-1972. (#CBA-301).
4. Kim, Y. et al. (2009). Cyclin-dependent kinase 2-associating protein 1 commits murine embryonic stem cell differentiation through retinoblastoma protein regulation. J. Biol. Chem. 284:23405-23414 (#CBA-301).
5. Yue, Y. et al. (2008). A single intravenous injection of adeno-associated virus serotype-9 leads to whole body skeletal muscle transduction in dogs. Mol. Ther. 16(12):1944-1952 (#CBA-301).
6. Ghosh, A. et al. (2007). Efficient whole-body transduction with trans-splicing adeno-associated viral vectors. Mol. Ther. 15(6):1220 (#CBA-301).
7. Shi, X. et al. (2007). The oncogenic potential of a prostate cancer-derived androgen receptor mutant. The Prostate 67(6):591-602 (#CBA-301).