For angiogenesis to occur, endothelial cells must escape their stable location and break through the basement membrane. Cells migrate toward an angiogenic stimulus that may be released from nearby tumor cells. These cells proliferate to form new blood vessels.
Our Endothelial Tube Formation Assay (In Vitro Angiogenesis) provides an easy, robust system to assess angiogenesisin vitro. The ECM gel matrix very closely resembles an in vivo environment.
Figure 1: HUVEC Tube Formation on ECM Gel. HUVEC cells from a standard tissue culture plate were incubated on an ECM gel. After several hours, tube formation can be visualized under a light microscope.