Cell adhesion is a complex process involved in migration/invasion, embryogenesis, wound healing and tissue remodeling. Cells adhere to the extracellular matrix, forming complexes with cytoskeleton components that can affect cell motility, differentiation, proliferation, and survival.
Our CytoSelect™ 48-Well Cell Adhesion Assays provide a fully quantitative method for the evaluation of cell adhesion. The 48-well plate is precoated with your choice of single ECM protein in each of the first 5 rows, with the last row provided as a negative control. Just follow a simple protocol:
Cells are seeded onto the substrate.
Adherent cells attach, while non-adherent cells are washed away.
Adherent cells are quantified on a standard plate reader or fluorometer.
Figure 1: CytoSelect™ 48-Well Cell Adhesion Assay, ECM Array. Serum starved cells from three different cell lines were allowed to attach to the ECM-coated 48-well plate for 1 hr at 100,000 cells/well. Adherent cells were stained according to the assay protocol. BSA = Bovine Serum Albumin. VN = Vitronectin. LN = Laminin. FN = Fibronectin. Col = Collagen.