The OxiSelect™ Hydrogen Peroxide Assay Kit is a quantitative assay for measuring aqueous peroxides and lipid peroxides. For aqueous samples, sorbitol first converts peroxide to a peroxyl radical, which oxidizes Fe+2 into Fe+3. For lipid samples, peroxide converts Fe+2 into Fe+3 directly. Then Fe+3 reacts with an equal molar amount of xylenol orange in the presence of acid to create a purple product that absorbs maximally between 540-600 nm. The antioxidant BHT is provided to prevent further undesirable chain peroxidation. The peroxide content in unknown samples is determined by comparison with the predetermined H2O2 standard curve.
The OxiSelect™ Hydrogen Peroxide/Peroxidase Assay Kit is a sensitive quantitative fluorometric assay for hydrogen peroxide or peroxidase. In the presence of HRP, ADHP reacts with H2O2 in a 1:1 stoichiometry to produce highly fluorescent Resorufin. The Resorufin product can be easily read by a fluorescence microplate reader with an excitation of 530-560 nm and an emission of 590 nm, or for absorbance at 560 nm. Fluorescence values are proportional to the H2O2 or peroxidase levels within the samples. The H2O2 or peroxidase content in unknown samples is determined by comparison with its respective standard curve.
