Hydrogen Peroxide/Peroxidase Assay, Fluorometric

The OxiSelect™ Hydrogen Peroxide/Peroxidase Assay Kit is a sensitive quantitative fluorometric assay for hydrogen peroxide or peroxidase.   In the presence of HRP, ADHP reacts with H₂O₂ in a 1:1 stoichiometry to produce highly fluorescent Resorufin.  The Resorufin product can be easily read by a fluorescence microplate reader with an excitation of 530-560 nm and an emission of 590 nm, or for absorbance at 560 nm.  Fluorescence values are proportional to the H₂O₂ or peroxidase levels within the samples.  The H₂O₂ or peroxidase content in unknown samples is determined by comparison with its respective standard curve.

1. Kim, E.Y. et al. (2012). Sustained Activation of N-Methyl-D-Aspartate Receptors in Podoctyes Leads to Oxidative Stress, Mobilization of Transient Receptor Potential Canonical 6 Channels, Nuclear Factor of Activated T Cells Activation, and Apoptotic Cell Death. Mol. Pharmacol. 82: 728-737 (#STA-344).
2. Kim, E.Y. et al.(2012).Insulin Increases Surface Expression of TRPC6 Channels in Podocytes: Role of NADPH Oxidases and Reactive Oxygen Species. Am J Physiol Renal Physiol. 302:F298-F307.(#STA-344)