Phagocytosis Assays

Phagocytosis can be assayed by measuring the engulfment of a cell "substrate". The most common substrates used in phagocytosis assays are erythrocytes (RBCs) and zymosan particles. However, traditional assays require tedious cell counting under a microscope.

Our CytoSelect™ 96-Well Phagocytosis Assays provide a more accurate, user-friendly, high-throughput alternative to the standard phagocytosis assay. The assay may be adapted for use with 24-well or 48-well plates.

1. Pierce, L.M. et al. (2012). Effect Of Heavy Metal Tungsten Alloy Particles On Oxidative Product Formation And Phagocytosis By Lung Macrophages. Am. J. Respir. Crit. Care Med. 185:A4666 (#CBA-224).
2. Lee, J.K. et al. (2011). Regulator of G-Protein Signaling-10 Negatively Regulates NF-kB in Microglia and Neuroprotects Dopaminergic Neurons in Hemiparkinsonian Rats. J. Neurosci. 31:11879-11888 (#CBA-220).
3. Winnicka, B. et al. (2010). CD13 is Dispensable for Normal Hematopoiesis and Myeloid Cell Functions in the Mouse. J. Leukoc. Biol. 10.1189/jlb.0210065(#CBA-220).
4. Dowling, D.J. et al. (2010). Major Secretory Antigens of the Helminth Fasciola Hepatica Activate a Suppressive Dendritic Cell Phenotype that Attenuates Th17 Cells but Fails to Activate Th2 Immune Responses. Infect. Immun. 78:793-801(#CBA-220).
5. Hamilton, C.M. et al. (2009). Fasciola Hepatica Tegumental Antigen Suppresses Dendritic Cell Maturation and Function. Infect. Immun. 77:2488-2498 (#CBA-220).
6. Polancec, D.S.et al.(2012). Azithromycin Drives in Vitro GM-CSF/IL-4-Induced Differentiation of Human Blood Monocytes Toward Dendritic-like Cells with Regulatory Properties. J Leukoc Biol. 91:229-243.(#CBA-224)