Platinum Retroviral Packaging Cell Lines

Retroviral Expression and Packaging

Higher retroviral yields: average titer 10⁶ to 10⁷ infectious units/mL with transient transfection
Longer stability: up to 4 months in the presence of drug selection
Three versions: ecotropic (Plat-E), amphotropic (Plat-A), and pantropic (Plat-GP)

Selection Guide for Retroviral Packaging Cells by Target Cell Species

NOTE: Platinum Retroviral Packaging Cells are available for sale to academic, government and non-profit research laboratories. All other purchasers require a commercial license for all fields including research use. Please contact our Business Development department for license information.

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Product Name	Catalog Number	Size	Detection	Price
Platinum-A Retroviral Packaging Cell Line, Amphotropic	RV-102	1 vial	N/A	Select Country
Platinum-E Retroviral Packaging Cell Line, Ecotropic	RV-101	1 vial	N/A	Select Country
Platinum-GP Retroviral Packaging Cell Line, Pantropic	RV-103	1 vial	N/A	Select Country

Overview

Conventional cells used for retrovirus packaging, such as those based on NIH3T3 cells, have limited stability and produce relatively low yields of retrovirus, mainly due to the poor expression of retroviral structure proteins (gag, pol and env) in the cells.

The Platinum Retroviral Packaging Cell Lines are based on the 293T cell line. They exhibit longer stability and produce higher yields of retroviral structure proteins. Plat-A and Plat-E cells contain gag, pol and env genes, allowing retroviral packaging with a single plasmid transfection.

Suitability of Retroviral Packaging Cell Lines by Host
	Plat-A Cells (Amphotropic)	Plat-E Cells (Ecotropic)	Plat-GP Cells (Pantropic*)
Human	+++	N.S.	+++
Mouse	+++	+++	+++
Rat	+++	+++	+++
Monkey	+++	N.S.	+++
Cat	+++	N.S.	+++
Dog	+++	N.S.	+++
Hamster	+	N.S.	+++
Bird	N.S.	N.S.	+++
Fish	N.S.	N.S.	+++
Frog	N.S.	N.S.	+++
Insect	N.S.	N.S.	+++
Mollusk	N.S.	N.S.	+++
*Retrovirus must be packaged with a pantropic envelope protein such as VSVG N.S. = Not Suitable

Table: Selection Guide for Platinum Retroviral Packaging Cells by Target Host.

Figures

Figure 1: High Retroviral Yields with Plat-E cells. NIH3T3 cells and mouse ProB Ba/F3 cells.

Figure 1

Figure 2: High Retroviral Yields with Plat-E cells. NIH3T3 cells were infected with GFP retrovirus supernatant produced in Plat-E cells after transfection with pMX-GFP.

Figure 2

Product Name	Catalog Number	Manual / Data Sheet	MSDS
Platinum-A Retroviral Packaging Cell Line, Amphotropic	RV-102	Download	Download
Platinum-E Retroviral Packaging Cell Line, Ecotropic	RV-101	Download	Download
Platinum-GP Retroviral Packaging Cell Line, Pantropic	RV-103	Download	Download

Recent Product Citations

Anantpadma, M and Vrati S.(2012) siRNA-Mediated Suppression of Japanese Encephalitis Virus Replication in Cultured Cells and Mice. J.Antimicrob Chemother. 67:444-451. (#RV-101)
Lahti, A.L. et al. (2012). Model for Long QT Syndrome Type 2 Using Human iPS Cells Demonstrates Arrhythmogenic Characteristics in Cell Culture. Dis. Model. Mech. 5:220-230. (#RV-101)
Tep, C. et al.(2012).Brain-derived Neurotrophic Factor (BDNF) Induces Polarized Signaling of Small GTPase (Rac1) Protein at the Onset of Schwann Cell Myelination through Partitioning-defective 3 (Par3) Protein. J.Biol Chem. 215:374-383.(#RV-101)
Anantpadma, M and Vrati S.(2012) siRNA-Mediated Suppression of Japanese Encephalitis Virus Replication in Cultured Cells and Mice. J.Antimicrob Chemother. 67:444-451.(#RV-101)
Huang, H.P. et al. (2011). Epithelial Cell Adhesion Molecule (EpCAM) Complex Proteins Promote Transcription Factor-mediated Pluripotency Reprogramming. J. Biol. Chem. 286:33520-33532 (#RV-101).
Lee, J-H. et al. (2010). Epigallocatechin-3-Gallate Inhibits Osteoclastogenesis by Down-Regulating c-fos Expression and Suppressing the Nuclear Factor k-B Signal. Mol. Pharmacol. 77:17-25 (#RV-101)
Li, W. et al. (2009). Rb/Cdk2/Cdk4 Triple Mutant Mice Elicit an Alternative Mechanism for Regulation of the G1/S Transition. PNAS 106:486-491. (#RV-101)
Huse, J.T. et al. (2009). The PTEN-Regulating MicroRNA Mir-26a is Amplified in High-Grade Glioma and Facilitates Gliomagenesis in Vivo. Genes & Dev. 23:1327-1337. (#RV-101)
Hinrichs, C.S. et al. (2009). Adoptively Transferred Effector Cells Derived from Naive Rather than Central Memory CD8+ Cells Mediate Superior Antitumor Immunity. PNAS 106:17469-17474.(#RV-101)
Cui, G. et al. (2009). Berberine Differentially Modulates the Activities of ERK, p38 MAPK, and JNK to Suppress Th17 and Th1 T Cell Differentiation in Type 1 Diabetic Mice. J. Biol. Chem. 284:28420-28429 (#RV-101).
Rice, K.L. et al. (2009). Comprehensive Genetic Screens Identify a Role for PLZR-RARalpha as a Positive Regulator of Cell Proliferation via Direct Regulation of c-myc. Blood 114:5499-5511 (#RV-101).
Qiao, Y. et al. (2011). FOXQ1 Regulates Epithelial-Mesenchymal Transition in Human Cancers. Cancer Res. 71:3076-3086 (#RV-102)
Hudecek, M. et al. (2010). The B-cell Tumor-associated Antigen ROR1 can be Targeted with T-Cells Modified to Express a ROR1-specific Chimeric Antigen Receptor. Blood 116:4532-4541 (#RV-102)
Oda, Y. et al. (2010). Induction of Pluripotent Stem Cells from Human Third Molar Mesenchymal Stromal Cells. J. Biol. Chem. 285:29270-29278 (#RV-102)
Wu, Y. et al. (2009). Imporved Coinfection with Amphotropic Pseudotyped Retroviral Vectors. J. Biochem. Biotechnol. 10.1155/2009/901079. (#RV-102)
Cavnar, P.J. et al. (2011). Hax1 Regulates Neutrophil Adhesion and Motility through RhoA. J. Cell Biol. 193:465-473 (#RV-103)

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