Cell Biolabs’ Urea Assay Kit is based on the Berthelot reaction. Urea is first degraded into ammonia and carbon dioxide, which further reacts with an alkaline developer to produce a blue-green colored product that can be measured with a standard spectrophotometric plate reader at an optical density between 580-630 nm. Each kit provides sufficient reagents to perform up to 192 assays, including blanks, urea standards and unknown samples.
Figure 1: Urea Assay Standard Curve.
Figure 2: Human Urine Sample Dilutions Tested with the Urea Assay Kit.
Figure 3: Human Plasma and Serum Sample Dilutions Tested with the Urea Assay Kit.