Lentivirus-Associated p24 ELISA Kit

Measuring the HIV-1 p24 antigen is a long-established method for lentivirus quantitation. However, the traditional p24 ELISA detects both virus-associated p24 and free p24 generated by 293 cells during transient transfection. Free p24 can account for a substantial portion of total p24 in the supernatant. Therefore, the ELISA typically overestimates the quantity of lentivirus present.

Our QuickTiter™ Lentivirus Titer Kit (Lentivirus-Associated HIV p24) substantially minimizes this problem. A proprietary technology separates the lentivirus from free p24 in solution prior to running the ELISA portion of the assay.

1. Zhou, B. et al. (2012). Interactions Between β-Catenin and Transforming Growth Factor-β Signaling Pathways Mediate Epithelial-Mesenchymal Transition and Are Dependent on the Transcriptional Co-Activator cAMP-Response Element-Binding Protein (CREB)-Binding. J.Biol. Chem. 287: 7026-7038.
2. Nedelec, A.D. et al. (2012). Noonan Syndrome-Causing SHP2 Mutants Inhibit Insulin-like Growth Factor 1 Release Via Growth Hormone-Induced ERK Hyperactivation, which Contributes to Short Stature. PNAS. 109:4257-4262.
3. Lavender, H. et al .(2012). In Vitro Characterization of the Activity of PF-05095808, a Novel Biological Agent for Hepatitis C Virus Therapy. Antimicrob. Agents Chemother. 56:1364-1375.
4. Keck, Z.Y. et al. (2011). Mapping a Region of Hepatitis C Virus E2 that is Responsible for Escape from Neutralizing Antibodies and a Core CD81-Binding Region that Does Not Tolerate Neutralization Escape Mutations. J. Virol. 85:10451-10463.
5. Sanchez-Antequera, Y. et al. (2011). Magselectofection: an Integrated Method of Nanomagnetic Separation and Genetic Modification of Target Cells. Blood 117:e171-e181.
6. Tiedemann, R.E. et al. (2010). Kinome-wide RNAi Studies in Human Multiple Myeloma Identify Vulnerable Kinase Targets, Including a Lymphoid Restricted Kinase, GRK6. Blood 115:1594-1604.
7. Lin, R-J. et al. (2009). Distinct Antiviral Roles for Human 2'5'-Oligoadenylate Synthetase Family Members Against Dengue Virus Infection. J. Immunol. 183:8035-8043.
8. Joshi, M.B. et al. (2009). Extracellular Cadherin Repeat Domains EC1 and EC5 of T-Cadherin are Essential for its Ability to Stimulate Angiogenic Behavior of Endothelial Cells. FASEB J. 10.1096/fj.09-133611.
9. Sukumaran S. et al. (2009). Functional Characterization of the Human 1-Acylglycerol-3-Phosphate-O-Acyltransferase Isoform 10/glycerol-3-Phosphate Acyltransferase Isoform 3. J. Mol. Endocrinol. 42:469-478.
10. Keck, Z.-y. et al. (2009). Mutations in HCV E2 Located Outside the CD81 Binding Sites Lead to Escape from Broadly Neutralizing Antibodies but Compromise Virus Infectivity. J. Virol. 10.1128/JVI.00248-09.
11. Dyer, K. et al. (2008). Functionally Competent Eosinophils Differentiated Ex Vivo in High Purity from Normal Mouse Bone Marrow. J. Immunol. 181:4004-4009.
12. Escamilla-Hernandez, R. et al. (2008). Constitutively Active Protein Kinase A Qualitatively Mimics the Effects of Follicle-Stimulating Hormone on Granulosa Cell Differentiation. Mol. Endocrin. 22(8):1842-1852.